splB
168
spore photoproduct lyase, radical SAM enzyme
locus
BSU_13930
Molecular weight
39.79 kDa
pI
8.52
function
protection of spore DNA against photodamage
product
spore photoproduct lyase
essential
no
synonyms
splB, spl
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1533 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,461,770 → 1,462,798
Phenotypes of a mutant
sensitive to blue light-induced DNA damage [pubmed|30054368]
The protein
Catalyzed reaction/ biological activity
repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine in DNA --> thymidine dimer in DNA (according to UniProt)
Protein family
[wiki|Radical SAM superfamily] (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
uses the [4Fe-4S] cluster to reduce SAM
Structure
[PDB|4FHC] (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) [Pubmed|22761404]
[AF|P37956]
Expression and Regulation
Operons
genes
[gene|8B3B4B181D949BD9B05AD6FCA5B6C773DBD84018|splA]-[gene|B873AE29ACAA0714D5F2E8648449B3280353C8AA|splB]
description
[Pubmed|8021181]
regulation
expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16497325]
regulatory mechanism
[protein|8B3B4B181D949BD9B05AD6FCA5B6C773DBD84018|splA]: negative autoregulation, in [regulon|protein:8B3B4B181D949BD9B05AD6FCA5B6C773DBD84018|splA regulon]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190,8021181], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
BP130 (Δ''splB''::''spc''), available in [wiki|Fabian Commichau]'s and [wiki|Jörg Stülke]'s labs [pubmed|30054368]
BKE13930 (Δ[gene|B873AE29ACAA0714D5F2E8648449B3280353C8AA|splB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGCCACATCCTTTTC, downstream forward: _UP4_TTCACTTAAACGGGCTGTTG
BKK13930 (Δ[gene|B873AE29ACAA0714D5F2E8648449B3280353C8AA|splB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGCCACATCCTTTTC, downstream forward: _UP4_TTCACTTAAACGGGCTGTTG
References
Reviews
Original Publications
Structure of the spore photoproduct lesion in DNA
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Time of last update: 2024-12-20 11:58:52
Author of last update: Jstuelk