alsT
168
putative glutamine transporter
locus
BSU_18120
Molecular weight
50.14 kDa
pI
9.39
function
uptake of glutamine
product
putative glutamine transporter
essential
no
synonyms
alsT
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1115 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,938,925 → 1,940,322
The protein
Protein family
[wiki|Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family] (according to UniProt)
Structure
[PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 36% identity) [pubmed|30659158]
[AF|Q45068]
Paralogous protein(s)
[protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|glnT], [protein|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD], [protein|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]
[wiki|Localization]
cell membrane (according to Swiss-Prot), membrane [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]
description
[pubmed|22383849]
regulation
repressed in the absence of good nitrogen sources (glutamine or ammonium) ([gene|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]) [Pubmed|12823818]
repressed in the presence of glutamine ([protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]) [Pubmed|28835263]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|12823818], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, [pubmed|28835263], in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
Biological materials
Mutant
GP1888 (Δ[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]::tet), available in [wiki|Jörg Stülke]'s lab [pubmed|32743959]
BKE18120 (Δ[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC, downstream forward: _UP4_TAATATAAATATAAAACAAA
BKK18120 (Δ[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC, downstream forward: _UP4_TAATATAAATATAAAACAAA
References
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Time of last update: 2024-12-21 17:24:16
Author of last update: Jstuelk