yxjF
168
sporulation protein, 3-hydroxybutyrate dehydrogenase
locus
BSU_38970
Molecular weight
27.46 kDa
pI
7.89
function
3-hydroxybutyrate utilization
product
3-hydroxybutyrate dehydrogenase
essential
no
synonyms
yxjF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,000,539 → 4,001,312
The protein
Protein family
[wiki|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)
Structure
[PDB|2YZ7] (from Alcaligenes Faecalis 42% identity)
[AF|P42317]
Paralogous protein(s)
[protein|D945CAD7209CB94A7B9E23263476124B7E8853DD|yusZ]
[protein|0DD474462720CAEE2AE17017BD7CA385238EBC6F|yxbG], (35%)
Expression and Regulation
Operons
genes
[gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]-[gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]-[gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]-[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]
description
[Pubmed|10746760]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|12662922]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135,10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-B730 (yxjF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1729 NBRP B. subtilis, Japan]
GP1112 (spc), available in [wiki|Stülke] lab
BKE38970 (Δ[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAAACACCTTCCCAT, downstream forward: _UP4_TGACATAAAAAACACACTTG
BKK38970 (Δ[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAAACACCTTCCCAT, downstream forward: _UP4_TGACATAAAAAACACACTTG
References
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Time of last update: 2024-12-30 10:41:04
Author of last update: Jstuelk