rnjB
168
RNase J2
locus
BSU_16780
Molecular weight
56.67 kDa
pI
9.18
function
RNA processing and degradation
product
RNase J2
essential
no
synonyms
rnjB, ymfA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0595 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,749,418 → 1,751,085
Phenotypes of a mutant
growth advantage at low pressure and at 27°C at normal pressure (has been selected at low pressure) [Pubmed|26296725]
The protein
Catalyzed reaction/ biological activity
endoribonuclease, involved in processing of ''[gene|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|thrS]'' mRNA
Protein family
[wiki|metallo-beta-lactamase superfamily] (according to UniProt)
[wiki|RNase] J subfamily (according to UniProt)
Structure
[PDB|3ZQ4], RNase J1, 49% identity, 81% similarity [Pubmed|21893285]
[AF|O31760]
Paralogous protein(s)
[protein|3EB289D0F58A58C693AB588798EE66A731341999|rnjA]
[wiki|Localization]
cytoplasm, colocalizes with the ribosomes [Pubmed|27708634]
Expression and Regulation
Operons
genes
[gene|B73CE26E7AEFACFA82C67F3E7DBBB3F766F739CA|rnjB]
description
[Pubmed|24187087]
regulation
constitutive expression [Pubmed|24187087]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|24187087], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B114 (ymfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1113 NBRP B. subtilis, Japan]
GP1113 ([gene|B73CE26E7AEFACFA82C67F3E7DBBB3F766F739CA|rnjB]::miniTn10 spc), available in [wiki|Jörg Stülke]'s lab
GP1291 (Δ[gene|B73CE26E7AEFACFA82C67F3E7DBBB3F766F739CA|rnjB]::cat), available in [wiki|Jörg Stülke]'s lab
BKE16780 (Δ[gene|B73CE26E7AEFACFA82C67F3E7DBBB3F766F739CA|rnjB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAATCTATATCCTCCTAG, downstream forward: _UP4_TAATGACTGACTAAAGACCG
BKK16780 (Δ[gene|B73CE26E7AEFACFA82C67F3E7DBBB3F766F739CA|rnjB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAATCTATATCCTCCTAG, downstream forward: _UP4_TAATGACTGACTAAAGACCG
Expression vectors
GP1687, chromosomal expression of '' rnjB''-Strep::''kan'' in '' B. subtilis'', available in [wiki|Jörg Stülke]'s lab
GP1724, chromosomal expression of '' rnjB''-Strep::''spc '' in '' B. subtilis'', based on [wiki|pGP1389], available in [wiki|Jörg Stülke]'s lab
pGP1438: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab
pGP1440: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
FLAG-tag construct
GP1001 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP419 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1695 (in pHJS-105 [Pubmed|26110430]), expression of '' rnjB-sfGFP''::''spc'' under a xylose-inducible promoter in '' B. subtilis'', available in [wiki|Jörg Stülke]'s lab [Pubmed|27708634]
GP1699 (in [wiki|pBP43]), expression of '' rnjB-GFP''::''spc'' under the native promoter, available in [wiki|Jörg Stülke]'s lab [Pubmed|27708634]
labs
[wiki|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-22 21:40:21
Author of last update: Jstuelk