pucD
168
xanthine dehydrogenase
locus
BSU_32480
Molecular weight
80.25 kDa
pI
5.75
function
purine utilization
product
xanthine dehydrogenase
essential
no
synonyms
pucD, yurC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1529 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,336,263 → 3,338,500
The protein
Catalyzed reaction/ biological activity
H2O + NAD+ + xanthine --> H+ + NADH + urate (according to UniProt)
H2O + hypoxanthine + NAD+ --> H+ + NADH + xanthine (according to UniProt)
Protein family
xanthine dehydrogenase family (single member, according to UniProt)
Structure
[PDB|1RM6] (4-hydroxybenzoyl-CoA reductase from Thauera aromatica, 30% identity) [pubmed|15576037]
[AF|O32144]
Expression and Regulation
Operons
genes
[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
description
[Pubmed|12823818]
regulation
induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029039], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A964 (yurC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/964 NBRP B. subtilis, Japan]
BKE32480 (Δ[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATTCCCCTCCCTCAG, downstream forward: _UP4_CAATGGACATAAAAGAGGCC
BKK32480 (Δ[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATTCCCCTCCCTCAG, downstream forward: _UP4_CAATGGACATAAAAGAGGCC
References
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Time of last update: 2025-04-08 17:44:15
Author of last update: Melvin.boenninger