trmFO
168
flavoprotein, tRNA:m(5)U-54 methyltransferase, glucose-inhibited division protein
locus
BSU_16130
Molecular weight
47.90 kDa
pI
5.77
function
tRNA modification
product
tRNA:m(5)U-54 methyltransferase
essential
no
ec
2.1.1.74
synonyms
trmFO, ylyC, gid
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1206 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,685,812 → 1,687,119
The protein
Catalyzed reaction/ biological activity
catalyzes the folate-dependent C(5)-methylation of uridine at position 54 in the TpsiC loop of tRNA
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + FADH2 + uridine54 in tRNA --> (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine54 in tRNA + FAD + H+ (according to UniProt)
Protein family
MnmG family (with [protein|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA], according to UniProt)
[wiki|Cofactors]
FAD, methylene-THF [Pubmed|24228791]
Structure
[PDB|3G5S] (TrmFO from ''Thermus thermophilus'', 54% identity, 79% similarity) [Pubmed|19416846]
[AF|P39815]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|B6062575849EA65BB7B44A9723676885799E2759|trmFO]-[gene|89746E312F20C81BE3CFDFCE7D99B01C375C198B|codV]-[gene|F6B748621B312E0B5A0A0782F3944C3E8112D7A8|clpQ]-[gene|2A5A080273CB7698DFABB147F4143E90BBCA3B01|clpY]-[gene|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]
description
[Pubmed|22383849]
regulation
repressed during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|11331605]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|11331605], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7783641], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the intracellular concentration of CodY is about 2.5 myM (according to [PubMed|20408793])
Biological materials
Mutant
MGNA-B147 (gid::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1146 NBRP B. subtilis, Japan]
BKE16130 (Δ[gene|B6062575849EA65BB7B44A9723676885799E2759|trmFO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCACATCTCCTAGT, downstream forward: _UP4_TAGGTATTGATTGCAACCTG
BKK16130 (Δ[gene|B6062575849EA65BB7B44A9723676885799E2759|trmFO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCACATCTCCTAGT, downstream forward: _UP4_TAGGTATTGATTGCAACCTG
References
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Time of last update: 2024-12-22 03:53:42
Author of last update: Jstuelk