ansZ
168
L-asparaginase
locus
BSU_02690
Molecular weight
39.94 kDa
pI
6.15
function
utilization of asparagine
product
L-asparaginase
essential
no
ec
3.5.1.1
synonyms
ansZ, yccC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0252 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
290,915 → 292,042
The protein
Catalyzed reaction/ biological activity
H2O + L-asparagine --> L-aspartate + NH4+(according to UniProt)
Protein family
asparaginase 1 family (with [protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA], according to UniProt)
[wiki|Domains]
Asparaginase/glutaminase domain (aa 51-375) (according to UniProt)
Structure
[PDB|1ZCF] (from ''Pectobacterium atrosepticum scri1043'', 55% identity, 73% similarity)
[AF|O34482]
Paralogous protein(s)
[protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]
Expression and Regulation
Operons
genes
[gene|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ]
description
[Pubmed|11914346]
regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|11914346]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|11914346], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11914346], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP4247 (Δ[gene|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ]::''kan''), available in [wiki|Jörg Stülke]'s lab
GP4248 (Δ[gene|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ]::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE02690 (Δ[gene|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTTCTCTCCTTACA, downstream forward: _UP4_TGAAGAAAAGAAGGCGAATA
BKK02690 (Δ[gene|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTTCTCTCCTTACA, downstream forward: _UP4_TGAAGAAAAGAAGGCGAATA
References
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Time of last update: 2025-04-08 18:43:01
Author of last update: Jstuelk