nos

nos
168

nitric-oxide synthase

locus
BSU_07630
Molecular weight
38.59 kDa
pI
5.53
Protein length
363 aaSequenceBlast
Gene length
1092 bpSequenceBlast
function
production of nitric oxide
product
nitric-oxide synthase
essential
no
synonyms
nos, yflM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4362 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
836,653 → 837,744
The protein
Catalyzed reaction/ biological activity
NADPH-dependent oxidation of l-[metabolite|arginine] into l-[metabolite|citrulline] [Pubmed|25826316]
2 L-[metabolite|arginine] + 4 O2 + 3 reduced [flavodoxin] --> 5 H+ + 4 H2O + 2 L-[metabolite|citrulline] + 2 nitric oxide + 3 oxidized [flavodoxin] (according to UniProt)
Protein family
NOS family (single member, according to UniProt)
[wiki|Cofactors]
[metabolite|heme b] [Pubmed|20006999]
[metabolite|NADPH] [Pubmed|25826316]
[metabolite|FMN] or [metabolite|FAD] [Pubmed|21635694]
Structure
[PDB|2AMO] (loose dimer),  [PDB|1M7V] (bound to tetrahydrofolate and arginine)
[AF|O34453]
Expression and Regulation
Operons
genes
[gene|B51ADFB53CA54309D866BB089502639E9571238B|nos]-[gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK]
description
[Pubmed|22383849]
additional information
Note that the [gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL] is located between [gene|B51ADFB53CA54309D866BB089502639E9571238B|nos] and [gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK], but on the opposite strand
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[gene|B51ADFB53CA54309D866BB089502639E9571238B|nos]→[gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK]

2025-02-20 04:10:50

Jstuelk

77

6f866daa346d0c3646437eec9014b264b60c39c2

D61A8EF1776363AE8A71D2E6E92AB155B9390F1B

Biological materials
Mutant
MGNA-C254 (yflM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2252 NBRP B. subtilis, Japan]
BKE07630 (Δ[gene|B51ADFB53CA54309D866BB089502639E9571238B|nos]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACAAACTCTCCCCTTCAC,  downstream forward: _UP4_AACTATTTTTATCAAGATAA
BKK07630 (Δ[gene|B51ADFB53CA54309D866BB089502639E9571238B|nos]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACAAACTCTCCCCTTCAC,  downstream forward: _UP4_AACTATTTTTATCAAGATAA
References
Reviews
9818193
Original Publications
12220171,11856757,20006999,21599925,22119809,23943262,21310962,21921039,25194416,25826316,26062720,28074650,36327499,39956041

B51ADFB53CA54309D866BB089502639E9571238B

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Time of last update: 2025-03-02 19:01:53

Author of last update: Jstuelk