ndk
168
nucleoside diphosphate kinase
locus
BSU_22730
Molecular weight
16.82 kDa
pI
5.57
function
maintainance of an equilibrium between the concentrations of different nucleoside triphosphates
product
nucleoside diphosphate kinase
essential
no
ec
2.7.4.6
synonyms
ndk
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0105 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,381,354 → 2,381,803
Phenotypes of a mutant
inactivation of ''[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]'' reduces sporulation efficiency to 13.4% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
2'-deoxyribonucleoside 5'-diphosphate + ATP --> 2'-deoxyribonucleoside 5'-triphosphate + ADP (according to UniProt)
ribonucleoside 5'-diphosphate + ATP --> ribonucleoside 5'-triphosphate + ADP (according to UniProt)
Protein family
NDK family (single member, according to UniProt)
Structure
[PDB|1WKJ] (from ''Thermus thermophilus'', 59% identity, 82% similarity)
[AF|P31103]
Modification
phosphorylation on Thr-92 AND Ser-123 [Pubmed|17218307]
Expression and Regulation
Operons
genes
[gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]-[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]-[gene|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|hepT]-[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]
description
[pubmed|22383849]
additional information
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
Biological materials
Mutant
BKE22730 (Δ[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCTCCACCCCATCAT, downstream forward: _UP4_TAATCATTGTGAAAATAAGC
BKK22730 (Δ[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCTCCACCCCATCAT, downstream forward: _UP4_TAATCATTGTGAAAATAAGC
References
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Time of last update: 2024-10-07 05:08:34
Author of last update: Jstuelk