ndk

ndk
168

nucleoside diphosphate kinase

locus
BSU_22730
Molecular weight
16.82 kDa
pI
5.57
Protein length
149 aaSequenceBlast
Gene length
450 bpSequenceBlast
function
maintainance of an equilibrium between the concentrations of different nucleoside triphosphates
product
nucleoside diphosphate kinase
essential
no
ec
2.7.4.6
synonyms
ndk
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0105 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,381,354 → 2,381,803
Phenotypes of a mutant
inactivation of ''[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]'' reduces sporulation efficiency to 13.4% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
2'-deoxyribonucleoside 5'-diphosphate + ATP --> 2'-deoxyribonucleoside 5'-triphosphate + ADP (according to UniProt)
ribonucleoside 5'-diphosphate + ATP --> ribonucleoside 5'-triphosphate + ADP (according to UniProt)
Protein family
NDK family (single member, according to UniProt)
Structure
[PDB|1WKJ] (from ''Thermus thermophilus'', 59% identity, 82% similarity)
[AF|P31103]
Modification
phosphorylation on Thr-92 AND Ser-123 [Pubmed|17218307]
Expression and Regulation
Operons
genes
[gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]-[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]-[gene|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|hepT]-[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]
description
[pubmed|22383849]
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[gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]→[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]

2024-12-21 14:49:44

Jstuelk

133

4994bd02adb7701a42b6eebb6e0352c9f8a44e38

8B6317CB1523DAD0B462E932EA20388911064C0F

genes
[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]
description
[pubmed|22383849]
Open in new tab

[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]

2024-12-30 16:28:22

Jstuelk

79

e78049fbd9f94bf70690facd3b3da013daab20cb

D0313F4563B02CC62065820FCC46E3BC389E3D38

additional information
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
Biological materials
Mutant
BKE22730 (Δ[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCTCCACCCCATCAT,  downstream forward: _UP4_TAATCATTGTGAAAATAAGC
BKK22730 (Δ[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCTCCACCCCATCAT,  downstream forward: _UP4_TAATCATTGTGAAAATAAGC
References
17218307,26735940,33782055,36815589

B498ED03F19B7A73878D5C947C28C2A87CAB934D

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Time of last update: 2024-12-31 00:08:04

Author of last update: Jstuelk