rocE

rocE
168

amino acid permease

locus
BSU_40330
Molecular weight
51.47 kDa
pI
8.53
Protein length
Gene length
function
arginine, ornithine and citrulline uptake
product
amino acid permease
essential
no
synonyms
rocE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0833 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,142,675 → 4,144,078
The protein
Protein family
[wiki|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
Structure
[PDB|6F34] (from Geobacillus kaustophilus, 23% identity) [pubmed|29416041]
[AF|P39137]
Paralogous protein(s)
[protein|B6A920650919D5BBBD75CCA58C30A0D8ECE64DA3|ybgF], [protein|C7FEA1D9C7C96C39CEE83CE3F13D412DE6AFE10E|ydgF], [protein|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|aapA], [protein|1594BA83CED2DA9F86C1CE2A0DC6E1C9B665EACB|gabP], [protein|219586A3F378DC38EA076FD39B99D41136BDE722|alaP], [protein|423AEC9D260BE53AD22851D999244B89BEB3E84D|hutM], [protein|8C4A8961B2514A8AD531AFB104B10C0797D02AC2|yvbW], [protein|9AD0E43119BDC65D5F67CE55CD9417E1E6BAE058|aimB], [protein|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]
[wiki|Localization]
cell membrane (according to UniProt),  membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]-[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]-[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]
description
[Pubmed|7540694]
regulation
induced in the presence of arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]) [Pubmed|7565595]
induced in the presence of arginine, citrulline or ornithine (with ornithine as the molecular inducer) ([protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]) [pubmed|37343703]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]: activation, [Pubmed|7540694], in [regulon|protein:62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]: activation, [Pubmed|7540694], in [regulon|protein:BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|7540694], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
additional information
expression of the [gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]-[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]-[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF] operon is increased upon depletion of [gene|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA] (resulting from increased [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] expression) [http://www.nature.com/articles/nmicrobiol20157 Reference]
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[gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]→[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]

2024-09-20 20:16:10

Jstuelk

114

2736177DCAE8E1DC5951F68E1BB418B48A3DD8A0

67A3AD2158130CCC732BB0A6D29A52A60C79A4AD

Biological materials
Mutant
GP1690 Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::''tet'', available in [wiki|Jörg Stülke]'s lab
GP4156 (Δ[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]::''aphA3'', Δ[gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]::''ermC'', Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::''tet'',), available in [wiki|Jörg Stülke]'s lab
BKE40330 (Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACATTCTGCACCCCTTCAG,  downstream forward: _UP4_TAAAAACGTTGTCCGCAAGT
BKK40330 (Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACATTCTGCACCCCTTCAG,  downstream forward: _UP4_TAAAAACGTTGTCCGCAAGT
References
14651647,12618455,18763711,7540694,29416041

B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6

Page visits: 4708

Time of last update: 2024-10-04 14:37:48

Author of last update: Jstuelk