hbuT
168
sporulation protein, transporter for 3-hydroxybutyrate
locus
BSU_39000
Molecular weight
49.98 kDa
pI
8.9
function
3-hydroxybutyrate utilization
product
transporter for 3-hydroxybutyrate
essential
no
synonyms
hbuT, yxjC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2610 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,002,716 → 4,004,134
Phenotypes of a mutant
reduced uptake of 3-hydroxybutyrate during [wiki|sporulation] [Pubmed|26363016]
The protein
Catalyzed reaction/ biological activity
uptake of 3-hydroxybutyrate [Pubmed|26363016]
Protein family
[wiki|CitM transporter family (TC 2.A.11)] (according to UniProt)
Structure
[AF|P42314]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]-[gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]-[gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]-[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]
description
[Pubmed|10746760]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|12662922]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135,10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-B727 (yxjC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1726 NBRP B. subtilis, Japan]
BKE39000 (Δ[gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACACACATCCCCCGTTT, downstream forward: _UP4_TAGAAGAAAACGAAAGGGGC
BKK39000 (Δ[gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACACACATCCCCCGTTT, downstream forward: _UP4_TAGAAGAAAACGAAAGGGGC
References
Page visits: 4301
Time of last update: 2024-12-30 15:23:24
Author of last update: Jstuelk