rodA
168
SEDS family peptidoglycan glycosyltransferase, part of the [wiki|elongasome] for lateral [wiki|cell wall synthesis] and control of cell diameter
locus
BSU_38120
Molecular weight
43.12 kDa
pI
8.76
function
control of cell shape and elongation
product
SEDS family peptidoglycan glycosyltransferase
essential
yes
synonyms
rodA, ywcF, ipa-42d
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0772 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,912,332 → 3,913,513
Phenotypes of a mutant
essential [Pubmed|12682299]
CRISPRi knockdown mutant has a terminal phenotype of severe lysis [Pubmed|27238023]
The protein
Catalyzed reaction/ biological activity
principal peptidoglycan glycosyltransferase of the [wiki|elongasome] [Pubmed|27525505]
[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)
Protein family
[wiki|SEDS proteins] (shape, elongation, division, and sporulation) [Pubmed|27525505]
SEDS family (with [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW] and [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE], according to UniProt)
Structure
[PDB|6BAR] (from Thermus thermophilus, 26% identity) [pubmed|29590088]
[AF|P39604]
Paralogous protein(s)
[protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE], [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW]
[wiki|Localization]
integral membrane protein [Pubmed|21636744]
forms circumferentially moving patches [Pubmed|21636744]
localization depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [pubmed|26679002]
Expression and Regulation
Operons
genes
[gene|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA]
description
[Pubmed|9353933]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
Biological materials
Mutant
xylose-inducible CRISPRi knockdown mutant BEC38120 is available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Daniel_R._Zeigler BGSC]
labs
[wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
[wiki|David Rudner], Harvard Medical School, MA, USA [http://rudnerlab.med.harvard.edu/ homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-30 02:27:55
Author of last update: Jstuelk