putP
168
high affinity proline permease
locus
BSU_03220
Molecular weight
48.25 kDa
pI
8.62
function
proline uptake
product
high affinity proline permease
essential
no
synonyms
putP, ycgO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0591 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
347,150 → 348,571
Phenotypes of a mutant
no growth with proline as single source of carbon or nitrogen [Pubmed|21840319]
The protein
Catalyzed reaction/ biological activity
uptake of proline coupled to the uptake of sodium ions
Protein family
[wiki|sodium:solute symporter (SSF) (TC 2.A.21) family] (according to UniProt)
Structure
[PDB|2XQ2] (from ''Vibrio parahaemolyticus'', 26% identity) [Pubmed|21131949]
[AF|P94392]
Paralogous protein(s)
[protein|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]
description
(internal promoter) [Pubmed|21840319]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21840319,21964733,22139509], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
genes
[gene|1F548515402950572E7212901729B2480432D1B9|putB]-[gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]-[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]
description
[Pubmed|21840319,22139509]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR]: activation, [Pubmed|21840319,21964733,22139509], in [regulon|protein:C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR regulon]
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, displacement of [protein|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR] [Pubmed|21840319], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21840319,21964733,22139509], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
overexpressed in a [gene|64C6D783FF3F41C81B216F798A1DC8071345B1ED|pnpA] mutant [PubMed|14976255]
the [gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP] part of the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-B994 (ycgO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1993 NBRP B. subtilis, Japan]
available in [wiki|Erhard Bremer]'s lab [Pubmed|24142252]
BKE03220 (Δ[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCGCCATATAAATAC, downstream forward: _UP4_TAAAGATCGAAAGGAGGAGG
BKK03220 (Δ[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCGCCATATAAATAC, downstream forward: _UP4_TAAAGATCGAAAGGAGGAGG
References
Page visits: 6629
Time of last update: 2024-10-05 23:23:00
Author of last update: Melvin.boenninger