ydfS
168
required for wet heat resistance of spores
locus
BSU_05540
Molecular weight
26.96 kDa
pI
8.45
function
stabilization of spore inner membrane proteins against wet heat
product
unknown
essential
no
synonyms
ydfS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2323 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
601,019 → 601,726
Phenotypes of a mutant
reduced wet heat resistance [pubmed|37113233]
deficient in spore germination in response to AGFK [pubmed|37113233]
The protein
Protein family
[wiki|UPF0702 family] (according to UniProt)
Structure
[PDB|3C6F] ([protein|EBD0F966FD7B3662814D530064BD307561A5CB15|yetF], corresponds to aa 81 ... 230, 36% identity)
[AF|P96697]
Paralogous protein(s)
[protein|EBD0F966FD7B3662814D530064BD307561A5CB15|yetF]
[wiki|Localization]
inner spore membrane [pubmed|37113233]
Expression and Regulation
Operons
genes
[gene|B37938C639FC87DAA72AA82998DA8DF25E63CF37|ydfS]
description
[Pubmed|16497325]
regulation
expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16497325]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|16497325], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
MGNA-C156 (ydfS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2154 NBRP B. subtilis, Japan]
BKE05540 (Δ[gene|B37938C639FC87DAA72AA82998DA8DF25E63CF37|ydfS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATGGCGCTCCCCGC, downstream forward: _UP4_AAGGAATGAAGGAACAATCA
BKK05540 (Δ[gene|B37938C639FC87DAA72AA82998DA8DF25E63CF37|ydfS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATGGCGCTCCCCGC, downstream forward: _UP4_AAGGAATGAAGGAACAATCA
References
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Time of last update: 2024-12-19 11:40:42
Author of last update: Jstuelk