ezrA
168
negative regulator of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] ring formation
locus
BSU_29610
Molecular weight
64.82 kDa
pI
4.76
function
control of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] ring formation
product
[protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ]-interacting protein, member of the [wiki|divisome]
essential
no
synonyms
ezrA, ytwP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4477 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,029,729 → 3,031,417
Phenotypes of a mutant
altered localization of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] and [protein|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1] [Pubmed|25403286]
growth defects, elongated cells [Pubmed|25403286]
the [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] mutation is synthetically lethal with a [gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF] mutation [Pubmed|24218584]
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] and [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] mutations are synthetically lethal, this can be suppressed by a [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ]-K86E mutant protein [pubmed|33737746]
depletion of ''[gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]'' and deletion of ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' have a strong synthetic defect in [wiki|cell division] [Pubmed|24825009]
an [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] [gene|9892E5BFE90EC934A4047795BC4B328656128C09|yrrS] double mutant has a severe growth defect [pubmed|39185233]
an [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] [gene|00A42FDB559BE2647CD682180420A435E2E7035F|facZ] double mutant has a severe growth defect [pubmed|39185233]
an [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] [gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH] double mutant has a severe growth defect [pubmed|39185233]
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The protein
Catalyzed reaction/ biological activity
inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] polymerization [Pubmed|25403286]
recruits [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1]]] to the division septum [Pubmed|25403286]
activates [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] [Pubmed|25845974]
Protein family
EzrA family (single member, according to UniProt)
Structure
[PDB|4UXV] (the 60 kDa cytoplasmic domain) [Pubmed|25403286]
[AF|O34894]
Effectors of protein activity
degradation of [protein|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] involves [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] [Pubmed|24222488]
[wiki|Localization]
cell membrane (integral) [Pubmed|10449747]
1 transmembrane domain at the N-terminus [Pubmed|25403286]
Expression and Regulation
Operons
genes
[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]
description
[Pubmed|9387221]
regulation
constitutively expressed [Pubmed|23701187]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15317798], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
degradation of [protein|search|EzrA] involves [protein|search|FtsH] [PubMed|24222488]
Biological materials
Mutant
MGNA-A428 (ytwP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/428 NBRP B. subtilis, Japan]
1A805 ( ''ezrA''::''kan''), [Pubmed|15101985], available at [http://bgsc.org BGSC]
1A1279 (''ezrA''::''spec''), [Pubmed|10449747], available at [http://bgsc.org BGSC]
1A1280 (''ezrA-gfp''), [Pubmed|10449747], available at [http://bgsc.org BGSC]
BKE29610 (Δ[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGAGCCCCCTTGCTG, downstream forward: _UP4_TAGATAATCACGACCATGAA
BKK29610 (Δ[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGAGCCCCCTTGCTG, downstream forward: _UP4_TAGATAATCACGACCATGAA
References
Reviews
Original Publications
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Time of last update: 2024-09-10 03:58:06
Author of last update: Jstuelk