kinA
168
two-component sensor kinase, phosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F], part of the [wiki|phosphorelay]
locus
BSU_13990
Molecular weight
68.99 kDa
pI
5.49
function
initiation of sporulation
product
two-component sensor kinase
essential
no
synonyms
kinA, spoIIF, spoIIJ, gsiC, scoD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5805 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,470,026 → 1,471,846
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]
mainly active in the older, inner regions of a colony (with [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|kinB]) [Pubmed|21097618]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
three tandem [wiki|PAS domain]s in the N-terminal region of KinA, the [wiki|second PAS] domain is the major N-terminal determinant of KinA dimerization [Pubmed|23504013]
the first [wiki|PAS domain] is required for NAD(+) binding [Pubmed|23599347]
3 [wiki|PAS domain]s (aa 3-73, aa 140-214, aa 265-335) (according to UniProt)
2 [wiki|PAC domain]s (aa 77-116, aa 218-255) (according to UniProt)
[wiki|Histidine Kinase domain] (aa 402-606) (according to UniProt)
Structure
[PDB|2VLG] (PAS domain)
[AF|P16497]
Modification
autophosphorylation on a His residue
Effectors of protein activity
interaction with [protein|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|pxpB] or [protein|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda] inhibits autophosphorylation of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA] [Pubmed|9334321,15023339]
autophosphorylation is inhibited by [protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|sivA] and [protein|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA] [Pubmed|23335417]
activated by interaction with NAD(+) that indicated poor respiratory activity of the cell [Pubmed|23599347], this has been falsified [pubmed|28449380]
[wiki|Localization]
cytoplasm
Expression and Regulation
Operons
genes
[gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]
description
[Pubmed|1569009]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|9644251]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|9644251], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
stringent response: positive regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|1569009], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
additional information
translation is repressed upon interaction of the mRNA with the [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA [pubmed|34478554]
promoter-up mutations or high artificial expression that render ''[wiki|kinA]'' independent from [wiki|SigH], allow [wiki|sporulation] at high salinity [Pubmed|26712348]
Biological materials
Mutant
BKE13990 (Δ[gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTTTGCA, downstream forward: _UP4_TTTCCAAAAAAATAAAAACA
BKK13990 (Δ[gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTTTGCA, downstream forward: _UP4_TTTCCAAAAAAATAAAAACA
References
Reviews
Original Publications
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Time of last update: 2024-12-21 00:08:26
Author of last update: Jstuelk