tcyN
168
[metabolite|cystine] and [metabolite|cysteinopantetheine] [wiki|ABC transporter ]([metabolite|ATP]-binding protein)
locus
BSU_29340
Molecular weight
29.41 kDa
pI
8.84
function
[metabolite|cystine] and [metabolite|cysteinopantetheine] uptake
product
[metabolite|cystine] and [metabolite|cysteinopantetheine] [wiki|ABC transporter ]([metabolite|ATP]-binding protein)
essential
no
synonyms
tcyN, hisP, ytmN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1126 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,004,346 → 3,005,125
The protein
Catalyzed reaction/ biological activity
hydrolysis of [metabolite|ATP] for the uptake of [metabolite|cystine] and [metabolite|cysteinopantetheine] [pubmed|39194188]
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
[wiki|ABC transporter domain] (aa 2-239) (according to UniProt)
Structure
[PDB|2Q0H] (complex with ADP-Mg2+, Geobacillus stearothermophilus, 54% identity )
[AF|O34900]
Paralogous protein(s)
[protein|B453986691E1231A1C565D469A2A60EBFF2F6BD3|tcyC], [protein|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO], [protein|0D5BC94E21D51373A50D61CE3D0895B3DB28F6B3|pstBB], [protein|0F8048267EC038D8CF673317D969BA27735473A7|yvrO], [protein|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|glnQ], [protein|6882EF93EC82872B25C88104F62D6603D2514890|artR], [protein|434BCED6953BE07D592AC229264C5AD0D04CBAF6|pstBA]
[wiki|Localization]
attached to the membrane via [protein|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[protein|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM] [Pubmed|10092453]
Expression and Regulation
Operons
genes
[gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
description
[Pubmed|15668000]
regulation
induced in the presence of methionine and taurine [Pubmed|11390694]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16109943], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
[protein|741156D495BE3857683C8A0390764EAD83845ABC|ascR]: activation, [Pubmed|16109943], in [regulon|protein:741156D495BE3857683C8A0390764EAD83845ABC|ascR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15272571], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP4468 (trpC2 Δ[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]::neo), available in [wiki|Jörg Stülke]'s lab
GP4676 (trpC2 Δ[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]::lox72), available in [wiki|Jörg Stülke]'s lab
GP4659 (trpC2 Δ[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]::tet), available in [wiki|Jörg Stülke]'s lab [pubmed|39194188]
BKE29340 (Δ[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTATGGATATTTTTAATCT, downstream forward: _UP4_GAACACATAAAGGAGCCGGT
BKK29340 (Δ[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTATGGATATTTTTAATCT, downstream forward: _UP4_GAACACATAAAGGAGCCGGT
Expression system
GP4687 (trpC2 xkdE::Pxyl-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-ermC), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-19 21:07:09
Author of last update: Jstuelk