engA
168
GTPase essential for ribosome 50S subunit assembly
locus
BSU_22840
Molecular weight
48.61 kDa
pI
5.25
function
ribosome assembly
product
GTPase
essential
yes
synonyms
yphC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1160 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,390,206 → 2,391,516
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes GTP and readily exchanges GDP for GTP
participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit [Pubmed|27484475]
Protein family
[wiki|TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily] (according to UniProt)
[wiki|Domains]
2 EngA-type G domains (aa 4-167, aa 176-351) (according to UniProt)
KH-like domain (aa 352-436) (according to UniProt)
[wiki|Cofactors]
Mg2+ [pubmed|36444972]
Structure
[PDB|2HJG] (complex with GDP) [pubmed|16894162]
[PDB|5M7H] (complex with GMPPNP and PO4)
[AF|P50743]
Paralogous protein(s)
[protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]-[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]
description
[pubmed|22383849]
genes
[gene|5DA8B27AC6A0E0B4115512BD948C31964D387A8E|yphA]-[gene|448375DF74EB0482A6CD1ACFE04D117C1319E2B2|seaA]-[gene|910109496E4A41EC892996DEEB81971FCBD9CB05|ypzH]-[gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA]-[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]
description
[pubmed|22383849]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-A402 (yphC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/402 NBRP B. subtilis, Japan]
Expression vectors
for expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP846, available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Naotake Ogasawara], Nara, Japan
References
Reviews
Original Publications
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Time of last update: 2024-12-21 12:54:47
Author of last update: Jstuelk