rqcP
168
ribosome-associated quality control factor, stabilizes the P-site tRNA conformation, similar to heat shock protein Hsp15, processivity factor essential for poly-alanine synthesis during ribosome quality control elongation by [protein|003047E90159765F9F0E499FD760F95FFF4AC360|rqcH]
locus
BSU_00590
Molecular weight
9.58 kDa
pI
10.14
function
ribosome-associated quality control
product
ribosome-associated quality control factor, P-site tRNA stabilizing factor
essential
no
synonyms
yabO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1188 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
67,877 → 68,137
The protein
Catalyzed reaction/ biological activity
translocates the tRNA moiety from the A site to the P site of stalled 50S ribosomal subunits [pubmed|19013177]
required for the synthesis of the poly-Ala degron tail to resolve stalled ribosomes [pubmed|33259811]
Protein family
[wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
[wiki|Domains]
[wiki|S4 RNA-binding domain] (aa 1-62) (according to UniProt)
Structure
[PDB|3BBU] (from E. coli, 36% identity) [pubmed|19013177]
[AF|P37557]
Expression and Regulation
Operons
genes
[gene|D6B1837075ECBEE1FEAC704DDB6795336092897F|yabM]-[gene|52D48F22CB53421EE30970D34B4861E2F8A5893D|yabN]-[gene|B0CB19A3E3E84E12BF42BB050394C8A059A89359|rqcP]-[gene|035B5486A3887FC71ACC0A8FB43ED77DC44E12EA|yabP]-[gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]-[gene|FE0E60B875E72AEF5174A89C7AE5556FF59B19D5|divIC]-[gene|8EDB1BCEEE64FDAF1C9054300751FCB420658865|yabR]
description
[Pubmed|11283287]
regulation
expressed during sporulation ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|11283287]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,11283287], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|12207695], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|12207695], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
Biological materials
Mutant
MGNA-B916 (yabO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1915 NBRP B. subtilis, Japan]
BKE00590 (Δ[gene|B0CB19A3E3E84E12BF42BB050394C8A059A89359|rqcP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAATTTATCTAATCTCA, downstream forward: _UP4_TAGGCTTGTTCTAAAAAAAC
BKK00590 (Δ[gene|B0CB19A3E3E84E12BF42BB050394C8A059A89359|rqcP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAATTTATCTAATCTCA, downstream forward: _UP4_TAGGCTTGTTCTAAAAAAAC
References
Reviews
Original Publications
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Time of last update: 2024-11-20 18:46:39
Author of last update: Jstuelk