katA
168
main vegetative catalase 1
locus
BSU_08820
Molecular weight
54.57 kDa
pI
6.15
function
detoxification (degradation) of hydrogen peroxide
product
vegetative catalase
essential
no
ec
1.11.1.6
synonyms
katA, kat-19
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0753 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
959,535 → 960,986
Phenotypes of a mutant
increased sensitivity to oxidative stress [Pubmed|22174384]
increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage [Pubmed|24973075]
The protein
Catalyzed reaction/ biological activity
2 H2O2 --> 2 H2O + O2 (according to UniProt)
Protein family
[wiki|catalase family] (according to UniProt)
[wiki|Cofactors]
contains an iron-sulfur cluster
heme [pubmed|36191235]
Structure
[PDB|1SI8] (enzyme from Enterococcus faecalis, 68% identity)
[AF|P26901]
Modification
phosphorylated on Arg-366 [Pubmed|22517742]
Effectors of protein activity
catalase activity is enhanced upon interaction with [protein|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB] [pubmed|36191235]
Paralogous protein(s)
[protein|DA5EB526EE8AF41BE9B837F2C276432BC4183AA1|katX], [protein|03FE63D6AAC77D6CEB052D0BF578ACD048C772FB|katE]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
aggregates at elevated temperatures, aggregation is reduced by interaction with [protein|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB] [pubmed|36191235]
Expression and Regulation
Operons
genes
[gene|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA]
description
[Pubmed|22174384]
regulation
induced in the presence of hydrogen peroxide and paraquat ([protein|search|PerR]) [Pubmed|11532148,22174384]
regulatory mechanism
[protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: repression, [Pubmed|11532148], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]
Biological materials
Mutant
GP1726 [gene|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA]::xeo trpC2 available at Jörg Stülkes lab
GP1725 [gene|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA]::cat trpC2 available at Jörg Stülkes lab
BKE08820 (Δ[gene|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCTCTTGGAA, downstream forward: _UP4_TAATGGAGAAATGCAAAAAC
BKK08820 (Δ[gene|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCTCTTGGAA, downstream forward: _UP4_TAATGGAGAAATGCAAAAAC
References
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Time of last update: 2024-12-22 00:21:50
Author of last update: Jstuelk