yugI
168
similar to the C-terminal domain of E. coli polyribonucleotide phosphorylase and to four repeated domains at the N-terminus of E. coli ribosomal protein S1, has an RNA-binding surface
locus
BSU_31390
Molecular weight
14.14 kDa
pI
5.92
function
unknown
product
GSP13
essential
no
synonyms
yugI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1098 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,225,178 3,225,570
The protein
[wiki|Domains]
[wiki|S1 domain] (aa 8-77) (according to UniProt)
Structure
[PDB|2K4K] [pubmed|19152054]
[AF|P80870]
Paralogous protein(s)
[protein|8EDB1BCEEE64FDAF1C9054300751FCB420658865|yabR], Array
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI]
description
[Pubmed|9274030]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|yugI]' and '[protein|search|alaT]' [PubMed|20525796]
Biological materials
Mutant
GP3597 (Δ[gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI]::''cat''), available in [wiki|Jörg Stülke]'s lab
GP3308 (Δ[gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI]::''tet''), available in [wiki|Jörg Stülke]'s lab
GP3309 (Δ[gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI]::''tet'') (Δ[gene|8EDB1BCEEE64FDAF1C9054300751FCB420658865|yabR]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
MGNA-A620 (yugI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/620 NBRP B. subtilis, Japan]
BKE31390 ([gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATAACAACTCCTAAG, downstream forward: _UP4_TAAGCATGAAAAAAGCACCG
BKK31390 ([gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATAACAACTCCTAAG, downstream forward: _UP4_TAAGCATGAAAAAAGCACCG
GP3322 ([gene|search|''yugI-His''] ''spec''), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP3700 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-22 01:08:34
Author of last update: Jstuelk