cdaA
168
diadenylate cyclase, synthesis of [metabolite|c-di-AMP] in vegetative cells
locus
BSU_01750
Molecular weight
30.42 kDa
pI
8.07
function
synthesis of [metabolite|c-di-AMP]
product
diadenylate cyclase
essential
no
ec
2.7.7.85
synonyms
cdaA, ybbP, ybbQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1624 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
196,213 → 197,034
Phenotypes of a mutant
inactivation of ''[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]'' results in severe beta-lactam sensitivity [Pubmed|22211522]
a [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] [gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA] double mutant or [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] [gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS] [gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA] triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) [pubmed|28420751]
increased spontaneous mutagenesis [pubmed|36613897]
The protein
Catalyzed reaction/ biological activity
synthesis of [metabolite|c-di-AMP] from two molecules of [metabolite|ATP] [Pubmed|23192352]
2 [metabolite|ATP] --> [metabolite|c-di-AMP] + 2 [metabolite|diphosphate] (according to UniProt)
Protein family
adenylate cyclase family (with [protein|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS], according to UniProt)
[wiki|Domains]
contains a [wiki|DAC domain] involved in the synthesis of [metabolite|c-di-AMP] [Pubmed|21566650]
[wiki|DAC domain] (aa 82-242) (according to UniProt)
[wiki|Cofactors]
Mn2+ [pubmed|31118276,25605729]
Structure
[PDB|8OGM] (the [wiki|DAC domain]) [pubmed|39177700]
[PDB|7OLH], [PDB|7OJS] (complex of the [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] [wiki|DAC domain] and [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM])
[PDB|4RV7] (the [wiki|DAC domain] and C-terminal domain of CdaA from ''Listeria monocytogenes'' (aa 101 - 273), 65% identity) [Pubmed|25605729]
[PDB|6HVL] (the [wiki|DAC domain] and C-terminal domain of CdaA from ''Listeria monocytogenes'' (aa 101 - 273) in complex with c-di-AMP, 65% identity) [Pubmed|31118276]
[AF|Q45589]
Effectors of protein activity
the interaction with [protein|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR] controls the diadenylate cyclase activity of [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] [Pubmed|23192352]
the interaction with [protein|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR] inhibits the diadenylate cyclase activity of [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] (shown in S. aureus) [pubmed|30668586]
the interaction with [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM] inhibits the diadenylate cyclase activity of [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] ([pubmed|34678313]) under conditions of osmotic stress (shown in L. monocytogenes) [pubmed|32250026]
Paralogous protein(s)
[protein|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS]
[wiki|Localization]
cell membrane [Pubmed|26240071]
Expression and Regulation
Operons
genes
[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]-[gene|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM]-[gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS]
description
[Pubmed|23192352]
regulatory mechanism
GlmS ribozyme: RNA switch, in [regulon|other_regulator:GlmS ribozyme|GlmS ribozyme]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22211522], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
CdaA levels are increased at increased potassium concentrations [pubmed|28420751]
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
GP94 Δ[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]::spec, available in [wiki|Jörg Stülke]'s lab [pubmed|28420751]
GP997 Δ[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]::cat, available in [wiki|Jörg Stülke]'s lab [pubmed|23192352]
GP2790 Δ[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]::aphA3, available in [wiki|Jörg Stülke]'s lab
GP985 Δ[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]::''cat'', available in [wiki|Jörg Stülke]'s lab [pubmed|23192352]
GP2222 [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]::cat [gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS]::ermC ''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]''::''tet'', available in [wiki|Jörg Stülke]'s lab, the mutant is only viable on minimal medium at low potassium concentration [pubmed|28420751]
BKE01750 (Δ[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTCGTCCTCCAAGA, downstream forward: _UP4_CGCTGGTATTGGAGGGGCAA
BKK01750 (Δ[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTCGTCCTCCAAGA, downstream forward: _UP4_CGCTGGTATTGGAGGGGCAA
Expression vectors
expression of native ''cdaA'' in ''B. subtilis'': pGP1960 (in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
expression of ''cdaA''-Strep in ''B. subtilis'' suitable for [wiki|SPINE]: pGP1986 (in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab
IPTG inducible expression of ''cdaA''-Strep in ''E. coli'': pGP2564 (in [wiki|pGP574]), available in [wiki|Jörg Stülke]'s lab
IPTG inducible expression of His-''cdaA'' in ''E. coli'': pGP1970 (in pET19B), available in [wiki|Jörg Stülke]'s lab [pubmed|23192352]
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab. Respective plasmid: pGP1990 [pubmed|23192352]
FLAG-tag construct
GP1381 ''cdaA-3xFLAG ermC'' (based on [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab [pubmed|28420751]
lacZ fusion
GP1339 (cat) based on [wiki|pAC6], available in [wiki|Jörg Stülke]'s lab [pubmed|23192352]
labs
[wiki|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]
References
Reviews
Original Publications
Page visits: 7880
Time of last update: 2024-12-31 08:43:21
Author of last update: Jstuelk