guaD
168
guanine deaminase, general stress protein, important for survival of ethanol and paraquat stresses
locus
BSU_13170
Molecular weight
17.01 kDa
pI
5
function
deamination of guanine to xanthine, purine salvage and interconversion
product
guanine deaminase
essential
no
ec
3.5.4.3
synonyms
guaD, gde, yknA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0590 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,382,677 → 1,383,147
The protein
Catalyzed reaction/ biological activity
guanine + H+ + H2O --> NH4+ + xanthine (according to UniProt)
Protein family
[wiki|Cytidine and deoxycytidylate deaminase family] (according to UniProt)
[wiki|Domains]
[wiki|CMP/dCMP-type deaminase domain] (aa 1-132) (according to UniProt)
Structure
[PDB|1WKQ]
[AF|O34598]
Expression and Regulation
Operons
genes
[gene|AEEC5CAC20D3A182074332087FD3CDCCF05F28CC|guaD]
description
[Pubmed|11101664]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11101664], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-A752 (yknA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/752 NBRP B. subtilis, Japan]
BKE13170 (Δ[gene|AEEC5CAC20D3A182074332087FD3CDCCF05F28CC|guaD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCAATCCCTCCTGTCA, downstream forward: _UP4_TAAAAGGATCAGGCATGCGC
BKK13170 (Δ[gene|AEEC5CAC20D3A182074332087FD3CDCCF05F28CC|guaD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCAATCCCTCCTGTCA, downstream forward: _UP4_TAAAAGGATCAGGCATGCGC
References
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Time of last update: 2024-10-12 05:15:13
Author of last update: TPed