aspB
168
aspartate transaminase
locus
BSU_22370
Molecular weight
42.93 kDa
pI
5.3
function
biosynthesis of aspartate
product
aspartate transaminase
essential
no
ec
2.6.1.1
synonyms
aspB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,347,660 2,348,841
Phenotypes of a mutant
auxotrophic for aspartate and asparagine [pubmed|38483038,29995990], the auxotrophy for aspartate can be bypassed by derepression of the [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB] operon (inactivation of [gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [pubmed|38483038]
lyses on DSM dporulation medium due to the inhibition of GltT-mediated Asp transport by glutamate and resulting depletion of meso-diaminopimelate [pubmed|29995990]
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + L-aspartate --> L-glutamate + oxaloacetate (according to UniProt)
Protein family
[wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|1J32] (from ''Phormidium lapideum'', 45% identity, 66% similarity)
[AF|P53001]
Paralogous protein(s)
[protein|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT], [protein|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|patA]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]-[gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]-[gene|EE712C1EC9E9C371847C83F7D75C189B276CD4B6|panD]-[gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]-[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|22383849]
genes
[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|29995990,22383849]
genes
[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|22383849]
regulation
constitutive
Biological materials
Mutant
GP4250 (Δ[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::''neo''), available in [wiki|Jörg Stülke]'s lab
BKE22370 ([gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT
BKK22370 ([gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT
References
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Time of last update: 2024-12-22 00:08:35
Author of last update: Jstuelk