aspB

aspB
168

aspartate transaminase

locus
BSU_22370
Molecular weight
42.93 kDa
pI
5.3
Protein length
Gene length
function
biosynthesis of aspartate
product
aspartate transaminase
essential
no
ec
2.6.1.1
synonyms
aspB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,347,660 2,348,841
Phenotypes of a mutant
auxotrophic for aspartate and asparagine [pubmed|38483038,29995990], the auxotrophy for aspartate can be bypassed by derepression of the [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB] operon (inactivation of [gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [pubmed|38483038]
lyses on DSM dporulation medium due to the inhibition of GltT-mediated Asp transport by glutamate and resulting depletion of meso-diaminopimelate [pubmed|29995990]
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + L-aspartate --> L-glutamate + oxaloacetate (according to UniProt)
Protein family
[wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|1J32] (from ''Phormidium lapideum'', 45% identity, 66% similarity)
[AF|P53001]
Paralogous protein(s)
[protein|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT], [protein|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|patA]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]-[gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]-[gene|EE712C1EC9E9C371847C83F7D75C189B276CD4B6|panD]-[gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]-[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|22383849]
Open in new tab

[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]→[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]

2024-10-08 08:17:37

Jstuelk

59

3C14CF916926052D4B293230C8985ACC791FC72B

3C14CF916926052D4B293230C8985ACC791FC72B

genes
[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|29995990,22383849]
Open in new tab

[gene|search|ympA]→[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]

2024-09-28 05:06:46

Jstuelk

62

1da6fe75124a7fb6321a113190f25efae7809829

46F8DB4DFCCA60148B66F350A7F182AB4E0080EE

genes
[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|22383849]
regulation
constitutive
Open in new tab

[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]→[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]

2024-09-02 13:35:58

ghost

77

853e576eeb824705f6265e3321731a7e0894baa0

509E8FD6169FFC3F028F50E50BD5DF60375119E6

Biological materials
Mutant
GP4250 (Δ[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::''neo''), available in [wiki|Jörg Stülke]'s lab
BKE22370 ([gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT
BKK22370 ([gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT
References
15378759,28232482,26883633,29995990,38483038

AE802E1D4F827DED3A5D953C7D034E6A3554C45F

Page visits: 4482

Time of last update: 2024-10-09 06:36:15

Author of last update: Jstuelk