rsbT
168
PP2C activator, protein serine kinase, phosphorylates [protein|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS] and [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR], part of the [wiki|stressosome]
locus
BSU_04690
Molecular weight
14.21 kDa
pI
6.59
function
control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] activity
product
PP2C activator, protein serine kinase
essential
no
synonyms
rsbT, ycxT
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2172 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
520,606 521,007
The protein
Catalyzed reaction/ biological activity
phosphorylation of [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR], [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|rsbRB], [protein|5EEEDE60CE9E5CDAEBAB0E03AABDF1DF62F1F002|rsbRC], and [protein|979D7A45EAD97C99015029400A85795061BAA367|rsbRD] [Pubmed|21362065]
activation of [protein|0B8381D2C724DAA724E4AFC67000F12AD112D13D|rsbU] phosphatase activity [pubmed|38405867]
ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)
ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
Structure
[PDB|1TIL] (the [protein|C8B867758330355F44C63CC284DC7AA3061FB9F1|spoIIAB]-[protein|3936E91C062074BFE4284B54A8CC33F35F94F5EE|spoIIAA] complex of Geobacillus stearothermophilus, 28% identity) [pubmed|15236958]
[AF|P42411]
Effectors of protein activity
phosphorylated [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR] activates the kinase activity of [protein|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT] [Pubmed|23320651]
activity is stimulated by light in a [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|ytvA]-dependent manner [Pubmed|23416074]
Expression and Regulation
Operons
genes
[gene|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]-[gene|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS]-[gene|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]-[gene|0B8381D2C724DAA724E4AFC67000F12AD112D13D|rsbU]-[gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]
description
[Pubmed|8002610]
regulation
''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|20454630], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8002610], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
BKE04690 ([gene|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACACAGGATTGGTCGTTCA, downstream forward: _UP4_TGGCTTCGGTAGGAGGTAAG
BKK04690 ([gene|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACACAGGATTGGTCGTTCA, downstream forward: _UP4_TGGCTTCGGTAGGAGGTAAG
labs
[wiki|Bill Haldenwang], San Antonio, USA
[wiki|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]
[wiki|Rick Lewis], Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/r.lewis homepage]
References
Reviews
Original Publications
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Time of last update: 2024-10-09 01:00:35
Author of last update: Jstuelk