cggR
168
repressor of the glycolytic [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] operon, [wiki|DeoR family]
locus
BSU_33950
Molecular weight
37.23 kDa
pI
5.68
function
transcriptional regulator
product
central glycolytic genes regulator
essential
no
synonyms
cggR, yvbQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2390 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,482,752 3,483,774
Phenotypes of a mutant
suppresses the growth defect of [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] mutants on gluconeogenic substrates [Pubmed|16272399]
The protein
Catalyzed reaction/ biological activity
transcription repression of the glycolytic ''[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]'' operon
Protein family
SorC transcriptional regulatory family (with [protein|E9C4C569F18B3335DA9C7FB5B17C87D661E62669|deoR], according to UniProt)
[wiki|Domains]
DNA binding domain (H-T-H motif) (3756)
Structure
[PDB|8R3G] (complex with [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] operon operator DNA) [pubmed|38842936]
[PDB|3BXH] (in complex with [metabolite|fructose-6-P]) [pubmed|18554327]
[PDB|2OKG] (effector binding domain) [pubmed|18554327]
[PDB|7OYK] (complex of the DNA-binding domain with the operator DNA) [pubmed|34726169]
[AF|O32253]
Effectors of protein activity
[metabolite|fructose-1,6-bisphosphate] [Pubmed|38842936,29923648,12622823] and [metabolite|dihydroxyacetone-P] [pubmed|38842936], [metabolite|glucose-6-P] and [metabolite|fructose-6-P] [Pubmed|18554327] act as inducer and result in release of [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] from the DNA
[wiki|Localization]
cytoplasma [Pubmed|20572937]
Expression and Regulation
Operons
genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
regulation
expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] [Pubmed|11489127]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [Pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]
description
[Pubmed|11489127]
regulation
expression induced by glucose (10 fold) ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [Pubmed|12850135,12622823]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-A463 (yvbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/463 NBRP B. subtilis, Japan]
GP311 (in frame deletion), available in [wiki|Jörg Stülke]'s lab
SM-NB7 (cggR-spc), available in [wiki|Anne Galinier]'s and [wiki|Boris Görke]'s labs
BKE33950 ([gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTCAAACGTTCCTTC, downstream forward: _UP4_TAATCCCTCAATATAAATAT
BKK33950 ([gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTCAAACGTTCCTTC, downstream forward: _UP4_TAATCCCTCAATATAAATAT
Expression vectors
pGP705 (N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP504 (in [wiki|pAC7]), pGP509 (in [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
Reviews
Original Publications
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Time of last update: 2024-12-21 03:38:07
Author of last update: Jstuelk