secA

secA
168

preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel

locus
BSU_35300
Molecular weight
95.33 kDa
pI
5.34
Protein length
841 aaSequenceBlast
Gene length
2526 bpSequenceBlast
function
protein secretion
product
preprotein translocase subunit (ATPase)
essential
yes
synonyms
secA, div, div-341, ts-341
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0653 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,628,310  3,630,835
Phenotypes of a mutant
essential [Pubmed|12682299]
terminal phenotype of CRISPRi knockdown mutant is moderate bending and a cessation of growth [Pubmed|27238023]
The protein
Catalyzed reaction/ biological activity
ATP  ADP + Pi + preprotein translocation
required for membrane targeting of [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|divIVA] [Pubmed|24592260]
Protein family
SecA family (single member, according to UniProt)
[wiki|Domains]
nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
[wiki|Cofactors]
Mg
Structure
[PDB|1TF5] (open structure),  [PDB|2IBM], [PDB|3DL8] (structure of the ([protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|secY]-[protein|1007EA15FB7AF81A44400287DB68BCC497158ACF|secE]-[protein|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|secG])-[protein|AD7CD451B4AE638A719C91780339784A7FF0F248|secA] complex [Pubmed|18923516]
[AF|P28366]
Effectors of protein activity
anionic phospholipids, preprotein, [protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|secY], signal peptides (even when added in trans) [Pubmed|19924216]
[wiki|Localization]
mainly in the cytoplasm [pubmed|38474341]
a small fraction is statically associated with the cell membrane [pubmed|38474341]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]-[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]
description
[Pubmed|9882663]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882663], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]→[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]

2025-01-14 22:35:45

Bzhu

99

00efa3c81b1b91aca22f058bbac28569ff967366

43AB19862CD02CECE51182E4F38F545D7AEAC1D6

Biological materials
Mutant
BEC35300, a CRISPRi knockdown mutant, is available from the [http://bgsc.org/ Bacillus Genetic Stock Center]
References
Reviews
25212246,27890920,27799326
Original Publications
19933328,19924216,18923516,12873133,12242434,1385592,10816431,19850053,9880811,12218047,17981983,9882663,23484952,20574771,23167435,23794293,23852076,24592260,24786965,11021932,7851746,7894702,10074074,8497195,8440733,7706219,7642557,27336478,27238023,27795329,28041832,27802584,22354575,38474341

AD7CD451B4AE638A719C91780339784A7FF0F248

Page visits: 6067

Time of last update: 2025-01-14 14:52:58

Author of last update: Jstuelk