purA
168
adenylosuccinate synthetase
locus
BSU_40420
Molecular weight
47.51 kDa
pI
5.46
function
purine biosynthesis
product
adenylosuccinate synthetase
essential
no
ec
6.3.4.4
synonyms
purA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0104 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,155,433 4,156,725
The protein
Catalyzed reaction/ biological activity
GTP + IMP + L-aspartate --> GDP + 2 H+ + N6-(1,2-dicarboxyethyl)-AMP + phosphate (according to UniProt)
Protein family
adenylosuccinate synthetase family (single member, according to UniProt)
Structure
[PDB|1KJX] (from ''Escherichia coli k12'', 46% identity, 64% similarity) [Pubmed|11741996]
[AF|P29726]
Modification
phosphorylated on Arg-97 [Pubmed|22517742]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|AB4B8B7325A1BF444D841EF1BBBCEC2D506AE600|purA]
description
[Pubmed|1312531]
regulation
expression activated by glucose (4.9 fold) ([protein|search|CcpA]) [Pubmed|12850135]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, [Pubmed|7638212], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1312531], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|CA7C472698CB9B9D75573377F42CCEE055D73EDD|yycE]-[gene|AB4B8B7325A1BF444D841EF1BBBCEC2D506AE600|purA]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE40420 ([gene|AB4B8B7325A1BF444D841EF1BBBCEC2D506AE600|purA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCGTGCACCTCCGTT, downstream forward: _UP4_TAAATAGAATATGTCTGCAA
BKK40420 ([gene|AB4B8B7325A1BF444D841EF1BBBCEC2D506AE600|purA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCGTGCACCTCCGTT, downstream forward: _UP4_TAAATAGAATATGTCTGCAA
References
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Time of last update: 2024-10-11 22:07:22
Author of last update: Melvin.boenninger