guaB
168
IMP dehydrogenase
locus
BSU_00090
Molecular weight
52.82 kDa
pI
6.17
function
biosynthesis of GMP
product
IMP dehydrogenase
essential
no
ec
1.1.1.205
synonyms
guaB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0517 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
15,915 17,381
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
reduced expression of [gene|AC67305E4C165CC1D785E49B7CD8335346134376|guaA] suppresses the requirement of a [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] [gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] [gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB] triple mutant for branched chain amino acids, methionine and threonine [Pubmed|24163341]
The protein
Catalyzed reaction/ biological activity
Inosine 5'-phosphate + NAD+ + H2O --> xanthosine 5'-phosphate + NADH (according to UniProt)
Protein family
IMPDH/GMPR family (together with [protein|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]) (according to UniProt)
[wiki|Domains]
2 [wiki|CBS domain]s (aa 95-153, aa 157-214) (according to UniProt)
Structure
[PDB|3TSB] (from ''B. anthracis'', 80% identity) [pubmed|22788966]
[PDB|7OJ1] (in complex with Ap4A) [pubmed|35953658]
[AF|P21879]
Modification
phosphorylated (STY) [Pubmed|17726680]
Cys308 is S-cysteinylated after diamide stress [Pubmed|17611193], [Pubmed|17726680]
Cys308 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|22938038]
Effectors of protein activity
inhibition of enzymatic activity by (p)ppGpp during the [regulon|stringent response] [Pubmed|22981860,6111556]
binding of Ap4A to the [wiki|CBS domain]s results in octamer formation and inhibition of [protein|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB] [pubmed|35953658]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB]
description
[Pubmed|22383849]
regulation
activated during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: activation, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
Biological materials
Mutant
BKE00090 ([gene|AC67305E4C165CC1D785E49B7CD8335346134376|guaA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGTAAATCCCCCTCTT, downstream forward: _UP4_TAATAAATTGTTACAAATTA
BKK00090 ([gene|AC67305E4C165CC1D785E49B7CD8335346134376|guaA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGTAAATCCCCCTCTT, downstream forward: _UP4_TAATAAATTGTTACAAATTA
Expression vectors
purification from ''B. subtilis'' with an N-terminal Strep-tag, for [wiki|SPINE], (in [wiki|pGP380]): pGP901, available in [wiki|Jrg Stlke]'s lab
pGP2937 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jrg Stlke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-21 16:26:56
Author of last update: Jstuelk