spoIID
168
cell wall hydrolase (lytic transglycosylase), required for complete dissolution of the asymmetric septum
locus
BSU_36750
Molecular weight
37.25 kDa
pI
9.76
function
dissolution of the septal cell wall
product
lytic transglycosylase
essential
no
synonyms
spoIID, spoIIC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2385 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,776,722 3,777,753
The protein
Catalyzed reaction/ biological activity
degrades the glycan strands of the peptidoglycan into disaccharide units [Pubmed|20159959], enhances [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] activity [Pubmed|20159959]
required for proper localization of [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] (together with [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]) [Pubmed|23834622]
Structure
[PDB|4RWR] (from ''B. anthracis'', 54% identity, 74% similarity) [pubmed|27226615]
[AF|P07372]
[wiki|Localization]
cell membrane [Pubmed|20159959]
the complex of [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID], [protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM], and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] localizes at the leading edge of the mother cell engulfing membrane and is essential and rate limiting for membrane migration [Pubmed|20382772,12502745]
localization of the [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]-[protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]-[protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] complex to the septal membrane depends on [protein|D98D61CA651AAA415386B1B2B41562E5CF77DEF1|spoIIB] [pubmed|36066101]
Expression and Regulation
Operons
genes
[gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]
description
[Pubmed|3011962]
regulation
expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE], [wiki|SpoIIID]) [Pubmed|15699190,15383836]
repressed by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P binding to a box downstream of the promoter [pubmed|37485800]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [pubmed|37485800], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE36750 ([gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCTGCCTCCTGC, downstream forward: _UP4_TAGAAAAAGACGCTCATTGG
BKK36750 ([gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCTGCCTCCTGC, downstream forward: _UP4_TAGAAAAAGACGCTCATTGG
References
Reviews
Original Publications
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Time of last update: 2024-12-20 02:39:47
Author of last update: Jstuelk