alsS
168
acetolactate synthase
locus
BSU_36010
Molecular weight
61.95 kDa
pI
5.16
function
overflow metabolism
product
acetolactate synthase
essential
no
ec
2.2.1.6
synonyms
alsS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0028 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,709,628 → 3,711,340
The protein
Catalyzed reaction/ biological activity
H+ + 2 pyruvate --> (2S)-2-acetolactate + CO2 (according to UniProt)
Protein family
[wiki|TPP enzyme family] (according to UniProt)
[wiki|Cofactors]
Mg2 [Pubmed|25393087]
TPP [Pubmed|25393087]
FAD (according to UniProt)
Structure
[PDB|4RJJ] (complex wit TPP) [Pubmed|25393087]
[AF|Q04789]
Paralogous protein(s)
[protein|E6EDDB6D1EDA85D73A0B78A656511D38346A625B|ilvB], [protein|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]
Expression and Regulation
Operons
genes
[gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD]
description
[Pubmed|7685336]
regulation
induction by acetate ([protein|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]) [Pubmed|30039521,7685336]
expression is heterogeneous [pubmed|29809139]
regulatory mechanism
[protein|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]: activation, in the presence of acetate [Pubmed|7685336], in [regulon|protein:8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR regulon]
[protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]: repression, if the ratio NADH2/NAD is high [Pubmed|16428414], in [regulon|protein:B5EF521437323EF43F08E5EFDB5C798616CA499A|rex regulon]
stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript [Pubmed|20081037], in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7685336], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
1A979 ( ''alsS''::''cat''), [Pubmed|7685336], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A979&Search=1A979 BGSC]
BKE36010 (Δ[gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACACCCTCACTCCTTATT, downstream forward: _UP4_TAGCACTCTGCGCATCACGA
BKK36010 (Δ[gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACACCCTCACTCCTTATT, downstream forward: _UP4_TAGCACTCTGCGCATCACGA
lacZ fusion
pGP3327 (cat, based on [wiki|pAC5]]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-10-14 11:02:15
Author of last update: Jstuelk