iolX
168
scyllo-inositol 2-dehydrogenase
locus
BSU_10850
Molecular weight
37.33 kDa
pI
5.3
function
utilization of scyllo-inositol
product
scyllo-inositol 2-dehydrogenase
essential
no
synonyms
iolX, yuxD, yucG, yisS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0673 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,164,370 1,165,398
Phenotypes of a mutant
impaired growth with scyllo-inositol as the only carbon source [Pubmed|20133360]
The protein
Catalyzed reaction/ biological activity
NAD+ + scyllo-inositol --> H+ + NADH + scyllo-inosose (according to UniProt)
Protein family
[wiki|Gfo/Idh/MocA family] (according to UniProt)
[wiki|Cofactors]
NAD+ [pubmed|28693424]
Structure
[PDB|3EZY] (from Thermotoga maritima, 37% identity)
[AF|P40332]
Paralogous protein(s)
[protein|03BE6DA854612B3F6741E0E167AB310F0DE96285|yfiI], [protein|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC], [protein|484D17480DFAD3667E0EBC6D38854A7546A8DB46|yteT], [protein|920C5750CB95102B073C32874D512E8D636D2C7D|iolU], [protein|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG], [protein|942BAE9FA023DFCA06B6D26A856A72F2979E23FA|yrbE], [protein|7DFE74C751A67CCC84579D52005E1399B0A10166|iolW]
Expression and Regulation
Operons
genes
[gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]
description
[pubmed|22383849]
regulation
induced by scyllo-inositol [pubmed|28693424]
regulatory mechanism
[protein|17F5188958A415D8D09439E02E1D1E0661B5C760|iolQ]: repression, [pubmed|28693424], in [regulon|protein:17F5188958A415D8D09439E02E1D1E0661B5C760|iolQ regulon]
Biological materials
Mutant
MGNA-B191 (yisS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1190 NBRP B. subtilis, Japan]
BKE10850 ([gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCCCATCCTCTCCTTT, downstream forward: _UP4_TAAAAGCTAAGTATGTTGCA
BKK10850 ([gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCCCATCCTCTCCTTT, downstream forward: _UP4_TAAAAGCTAAGTATGTTGCA
References
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Time of last update: 2025-01-29 08:01:03
Author of last update: Jstuelk