gerM
168
lipoprotein, germination (cortex hydrolysis) and sporulation, required for the assembly of the basal platform of the [protein|search|SpoIIIA]-[protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] trans-envelope complex during sporulation
locus
BSU_28380
Molecular weight
39.41 kDa
pI
4.68
function
germination (cortex hydrolysis) and sporulation
product
[protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] recruitment factor
essential
no
synonyms
gerM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5401 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,902,002 2,903,102
Phenotypes of a mutant
inactivation of ''[gene|AA519F0D7DC94165C2CF5F7E230502D884898B71|gerM]'' reduces sporulation efficiency to 0.3% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
required for the assembly of the basal platform of the [protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]-[protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] transenvelope complex during [wiki|sporulation] [Pubmed|27381174]
required for the recruitment of [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] to the septal membranes on the forespore side [Pubmed|27381174]
Structure
[PDB|6GZ8] (first GerMN domain) [Pubmed|30266596]
[PDB|6GZB] (tandem GerMN domains) [Pubmed|30266596]
[AF|P39072]
[wiki|Localization]
lipoprotein, outer forespore membrane, localization depends on [protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH] and [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] [Pubmed|27381174]
Expression and Regulation
Operons
genes
[gene|AA519F0D7DC94165C2CF5F7E230502D884898B71|gerM]
description
[Pubmed|8969504]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|8969504,15699190,15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8969504,15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE28380 ([gene|AA519F0D7DC94165C2CF5F7E230502D884898B71|gerM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTTTTCCCTCCTAAGG, downstream forward: _UP4_TAAATTGGGATTTTTGATAT
BKK28380 ([gene|AA519F0D7DC94165C2CF5F7E230502D884898B71|gerM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTTTTCCCTCCTAAGG, downstream forward: _UP4_TAAATTGGGATTTTTGATAT
References
Reviews
Original Publications
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Time of last update: 2024-12-18 20:02:43
Author of last update: Jstuelk