gyrB
168
DNA gyrase (subunit B)
locus
BSU_00060
Molecular weight
71.32 kDa
pI
5.38
function
DNA supercoiling, control of [category|SW.3.1.1|replication] initiation, resolution of head-on [category|SW.3.1.1|DNA replication]-[category|SW.3.2.1|Transcription] conflicts
product
DNA gyrase (subunit B)
essential
yes
ec
5.99.1.3
synonyms
gyrB, novA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0187 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,867 6,783
Phenotypes of a mutant
essential [Pubmed|12682299]
enhanced colony wrinkling upon knockdown [pubmed|36069446]
The protein
Catalyzed reaction/ biological activity
ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)
decreases [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] association with the oriC and inhibits [category|SW.3.1.1|replication] initiation [pubmed|29396913]
Protein family
type II topoisomerase [protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB] family (together with [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]) (according to UniProt)
[wiki|Domains]
[wiki|TOPRIM domain] (aa 422-536) [Pubmed|9722641]
Structure
[PDB|4I3H] (from ''Streptococcus pneumoniae'', 55% identity) [Pubmed|23965305]
[PDB|9GBV] (E. coli DNA gyrase with chirally wrapped 217 bp DNA fragment) [pubmed|39589884]
[PDB|9GGQ] (E. coli DNA gyrase with chirally wrapped 217 bp DNA fragment in the presence of the fluoroquinolone moxifloxacin) [pubmed|39589884]
[AF|P05652]
Modification
phosphorylated on Ser-400 [Pubmed|20509597]
Effectors of protein activity
novobiocin binds to the ATP binding site of GyrB and inhibits ATP hydrolysis and enzymatic activity, this results in [category|SW.3.1.1|replication] overinitiation [pubmed|29396913,6282812,368801]
an R138L mutation confers novobiocin resistance [pubmed|29396913]
Paralogous protein(s)
[protein|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]
[wiki|Localization]
nucleoid-associated in actively growing cells [Pubmed|9539793]
Expression and Regulation
Operons
genes
[gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2987848], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
labs
[wiki|Dagmar Klostermeier], University of Mnster, Germany [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 01:17:44
Author of last update: Jstuelk