gyrB

gyrB
168

DNA gyrase (subunit B)

locus
BSU_00060
Molecular weight
71.32 kDa
pI
5.38
Protein length
638 aaSequenceBlast
Gene length
1917 bpSequenceBlast
function
DNA supercoiling, control of [category|SW.3.1.1|replication] initiation, resolution of head-on [category|SW.3.1.1|DNA replication]-[category|SW.3.2.1|Transcription] conflicts
product
DNA gyrase (subunit B)
essential
yes
ec
5.99.1.3
synonyms
gyrB, novA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0187 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,867  6,783
Phenotypes of a mutant
essential [Pubmed|12682299]
enhanced colony wrinkling upon knockdown [pubmed|36069446]
The protein
Catalyzed reaction/ biological activity
ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)
decreases [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] association with the oriC and inhibits [category|SW.3.1.1|replication] initiation [pubmed|29396913]
Protein family
type II topoisomerase [protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB] family (together with [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]) (according to UniProt)
[wiki|Domains]
[wiki|TOPRIM domain] (aa 422-536) [Pubmed|9722641]
Structure
[PDB|4I3H] (from ''Streptococcus pneumoniae'', 55% identity) [Pubmed|23965305]
[AF|P05652]
Modification
phosphorylated on Ser-400 [Pubmed|20509597]
Effectors of protein activity
novobiocin binds to the ATP binding site of GyrB and inhibits ATP hydrolysis and enzymatic activity, this results in [category|SW.3.1.1|replication] overinitiation [pubmed|29396913,6282812,368801]
an R138L mutation confers novobiocin resistance [pubmed|29396913]
Paralogous protein(s)
[protein|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]
[wiki|Localization]
nucleoid-associated in actively growing cells [Pubmed|9539793]
Expression and Regulation
Operons
genes
[gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2987848], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]→[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]

2024-10-02 04:14:54

Jstuelk

122

7cfa0ee36064fef3f7a603b125c665e7c80f35bd

A6120E836ADBF13F415EC7E80D314C2CE36E1D65

labs
[wiki|Dagmar Klostermeier], University of Mnster, Germany [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]
References
Reviews
16936698,29772727,33480441,33959387
Original Publications
17981983,9539793,2987848,19666507,17320901,20509597,23475963,23965305,9722641,27557712,27793284,12767818,368801,6282812,29396913,8940171,7590259,10944214,6248518,6243420,225110,17334374,30517674,31953321,33657379,36069446

AA42910B71348EFEA4165CFFCEF1E497897B9DDC

Page visits: 8029

Time of last update: 2024-10-06 05:20:13

Author of last update: Jstuelk