gltB
168
small subunit of [metabolite|glutamate] synthase
locus
BSU_18440
Molecular weight
54.78 kDa
pI
7.7
function
[metabolite|glutamate] biosynthesis
product
[metabolite|glutamate] synthase (small subunit)
essential
no
ec
1.4.1.13
synonyms
gltB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0493 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,008,572 2,010,053
Phenotypes of a mutant
auxotrophic for [metabolite|glutamate] [pubmed|38483038], can be bypassed by inactivation of [gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG] and [gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR] (as a result of [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB] derepression)[pubmed|38483038]
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The protein
Catalyzed reaction/ biological activity
2 L-[metabolite|glutamate] + [metabolite|NADP]+ --> [metabolite|2-oxoglutarate] + H+ + L-[metabolite|glutamine] + [metabolite|NADPH] (according to UniProt)
Protein family
glutamate synthase family (with [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA] and [protein|D1AF9DA2594D2E2A94DC207A870C8495954A7A17|yerD], according to UniProt)
[wiki|Domains]
nucleotide binding domain ([metabolite|NADP]) (299313)
[wiki|Cofactors]
two [metabolite|4Fe-4S] clusters [https://www.biorxiv.org/content/10.1101/2021.04.12.439528v1 reference]
Structure
[PDB|2VDC] (the [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[protein|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB] complex of ''Azospirillum brasiliense'') [Pubmed|18199747], note that B. subtilis [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA] is unable to form dimers, thus [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[protein|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB] exists as a heterodimer [pubmed|34931064]
[PDB|7MFT] (the [protein|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|gudB]6-([protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[protein|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB])6 complex) [pubmed|34931064]
[AF|O34399]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]
description
[Pubmed|12823818]
regulation
expressed in the presence of ammonium [Pubmed|11029411]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|11029411], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|87BCAE725B02860156D50E1783F6DB68510C811E|gltC]: activation, [Pubmed|2548995], in [regulon|protein:87BCAE725B02860156D50E1783F6DB68510C811E|gltC regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: indirect effect, in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2548995], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Other regulations
[protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]: translation inhibition
Biological materials
Mutant
BP123 (Δ[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::''ermC'') , available in [wiki|Fabian Commichau]'s lab
GP807 (Δ[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::''tet'') , available in [wiki|Jörg Stülke]'s lab
GP517 (Δ[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::''ermC''), available in [wiki|Jörg Stülke]'s lab
BKE18440 ([gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT, downstream forward: _UP4_TAAATAAAGGGGATTATCAT
BKK18440 ([gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT, downstream forward: _UP4_TAAATAAAGGGGATTATCAT
Expression vectors
pGP1119 (in [wiki|pGP380], for SPINE, expression in ''B. subtilis''), available in [wiki|Jörg Stülke]'s lab [pubmed|20933603]
pGP3031: IPTG inducible expression, purification in ''E. coli'' with C-terminal His-tag, in [wiki|pGP574] (Strep-tag omitted), available in [wiki|Jörg Stülke]'s lab
pGP3032: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [wiki|pGP574], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
see ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]
labs
[wiki|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
[wiki|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-09-10 07:54:49
Author of last update: Jstuelk