purR
168
transcription repressor of the pur operon
locus
BSU_00470
Molecular weight
31.08 kDa
pI
5.77
function
regulation of purine biosynthesis
product
transcription repressor of the pur operon
essential
no
synonyms
purR, yabI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0503 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
54,441 55,298
The protein
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
[wiki|Domains]
N-terminal winged helix-turn-helix domain [pubmed|34967415]
C-terminal effector binding domain [pubmed|34967415]
Structure
[PDB|1O57] (the apo-protein) [Pubmed|12837783]
[PDB|7RMW] (complex with ppGpp) [pubmed|34967415]
[AF|P37551]
Effectors of protein activity
PRPP acts as inducer, binding results in release of PurR from the operator [Pubmed|10919400]
(p)ppGpp acts as an anti-inducer, prevents binding of the inducer PRPP [pubmed|34967415]
Kinetic information
KD for ppGpp: 4.4 µM [pubmed|34967415]
Expression and Regulation
Operons
genes
[gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
description
[Pubmed|22383849]
regulation
negative autoregulation [Pubmed|7638212]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, [Pubmed|7638212], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
Biological materials
Mutant
BKE00470 ([gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCCCCAAAAT, downstream forward: _UP4_TTAAAGAATGGAGAGACAGA
BKK00470 ([gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCCCCAAAAT, downstream forward: _UP4_TTAAAGAATGGAGAGACAGA
References
Reviews
Original Publications
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Time of last update: 2024-12-21 06:21:42
Author of last update: Jstuelk