bsuRB
168
component of the BsuM DNA restriction system, similar to 5-methylcytosine-specific restriction enzyme
locus
BSU_06100
Molecular weight
39.88 kDa
pI
8.7
function
BsuM DNA restriction
product
protection against foreign DNA
essential
no
synonyms
ydiS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1401 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
660,596 661,627
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates (according to UniProt)
Structure
[PDB|6HZ4] (5-methylcytosine-specific restriction enzyme from E. coli, corresponds to aa 29 ... 223, 29% identity) [pubmed|31296862]
[AF|O34885]
Expression and Regulation
Operons
genes
[gene|4BD4CFB10700907DF1BBF3C9CFACAF573588E85F|bsuRA]-[gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|bsuRB]-[gene|D36B91B3C8EAFEB80A7395C62A50A5F80B8CC5C5|bsuRC]
description
[Pubmed|11751814]
Biological materials
Mutant
MGNA-C208 (ydiS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2206 NBRP B. subtilis, Japan]
DELTA0 (''ydiS trpC2''), available at BGSC
BKE06100 ([gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|bsuRB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT, downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
BKK06100 ([gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|bsuRB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT, downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
References
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Time of last update: 2025-02-02 13:33:47
Author of last update: Jstuelk