xlyB
168
N-acetylmuramoyl-L-alanine amidase
locus
BSU_12460
Molecular weight
33.71 kDa
pI
9.8
function
PBSX prophage-mediated lysis
product
N-acetylmuramoyl-L-alanine amidase
essential
no
ec
3.5.1.28
synonyms
xlyB, yjpB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,317,535 1,318,488
The protein
Catalyzed reaction/ biological activity
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
Protein family
[wiki|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)
[wiki|Domains]
contains a N-acetylglucosamine-polymer-binding [wiki|LysM domain] [Pubmed|18430080]
contains an amidase_2 domain (like [protein|C1EB8D05386C1B35B8002239F5247CC6AB25F786|blyA], [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA], [protein|D5612882F1887BE342EF7072E71502382AB7289F|cwlH], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB])
[wiki|N-acetylmuramoyl-L-alanine amidase domain] (aa 40-142) (according to UniProt)
[wiki|LysM domain] (aa 177-221) (according to UniProt)
Structure
[PDB|2L47] (from Bacillus phage Gamma, corresponds to aa 3 ... 166, 57% identity)
[AF|O34391]
Paralogous protein(s)
[protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA], [protein|D5612882F1887BE342EF7072E71502382AB7289F|cwlH], [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|xlyA]
[wiki|Localization]
secreted (according to UniProt)
Expression and Regulation
Operons
genes
[gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]
description
[pubmed|22383849]
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
BKE12460 ([gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT
BKK12460 ([gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT
References
Reviews
Original Publications
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Time of last update: 2025-04-06 07:03:58
Author of last update: Jstuelk