nasE
168
assimilatory nitrite reductase (subunit)
locus
BSU_03290
Molecular weight
11.76 kDa
pI
4.8
function
utilization of nitrite as nitrogen source
product
assimilatory nitrite reductase (subunit)
essential
no
ec
1.7.1.4
synonyms
nasE, nasBD, nirD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2146 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
355,412 355,732
The protein
Catalyzed reaction/ biological activity
2 H2O + 3 NADP+ + NH4+ --> 5 H+ + 3 NADPH + nitrite (according to UniProt)
2 H2O + 3 NAD+ + NH4+ --> 5 H+ + 3 NADH + nitrite (according to UniProt)
[wiki|Domains]
Rieske domain (aa 8-104) (according to UniProt)
[wiki|Cofactors]
NAD [Pubmed|11289299]
FeS cluster [pubmed|29292548]
Structure
[PDB|5BOK] (from Diaphorobacter sp., 30% identity)
[AF|P42436]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]-[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]-[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]
description
[Pubmed|7836289]
regulation
''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|10972836], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [PubMed|8799114,9765565], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7836289], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]
description
[Pubmed|9765565]
regulation
''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
Biological materials
Mutant
BKE03290 ([gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCATTCCTCCTCAAT, downstream forward: _UP4_TGATCAAAAGACCGGATCAC
BKK03290 ([gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCATTCCTCCTCAAT, downstream forward: _UP4_TGATCAAAAGACCGGATCAC
References
Reviews
Original Publications
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Time of last update: 2024-11-02 07:00:23
Author of last update: Jstuelk