pfkA
168
phosphofructokinase, glycolytic enzyme
locus
BSU_29190
Molecular weight
34.10 kDa
pI
6.14
function
catabolic enzyme in glycolysis
product
6-phosphofructokinase
essential
no
ec
2.7.1.11
synonyms
pfkA, pfk
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0205 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,986,588 2,987,547
Phenotypes of a mutant
essential according to [Pubmed|12682299], non-essential according to [Pubmed|23420519,20473954]
poor growth [pubmed|28189581]
poorly transformable [pubmed|28189581]
increased resistance to diamide [pubmed|37819171]
The protein
Catalyzed reaction/ biological activity
[metabolite|ATP] + β-D-[metabolite|fructose-6-P] --> [metabolite|ADP] + β-D-[metabolite|fructose-1,6-bisphosphate] + H+ (according to UniProt)
Protein family
phosphofructokinase type A (PFKA) family (single member, according to UniProt)
[wiki|Domains]
3 x nucleotide binding domain ([metabolite|ATP]) (2125), (154158), (171187)
[wiki|Cofactors]
Mg2+
Structure
[PDB|4A3S] [Pubmed|22198292]
[PDB|1MTO] (mutant, complex with [metabolite|fructose-6-P], ''Geobacillus stearothermophilus'')
[PDB|4PFK] (''Geobacillus stearothermophilus'')
[AF|O34529]
Modification
phosphorylated on Arg-233 [Pubmed|22517742]
Effectors of protein activity
Inhibited by [metabolite|citrate], [metabolite|PEP] (Hill Coefficient 3) and Ca2+ (competes with Mg2+) in ''B. licheniformes'' [Pubmed|4269800].
Inhibited by [metabolite|ATP] (competitively) and [metabolite|fructose-6-P] (non-competitively) in ''G. stearothermophillus'' [Pubmed|8136379]
Activated by [metabolite|GDP] and [metabolite|ADP] in lower concentrations (1 mM); above that inhibition, competing with the [metabolite|ATP] for the binding site (in ''G. stearothermophillus'') [Pubmed|7873536]
Activated by NH4+ [Pubmed|7873536]
Kinetic information
Allosteric Regulation (Reversible) [Pubmed|4269800]
[wiki|Localization]
cytoplasm (homogeneous) [Pubmed|29439991,16479537,27708634]
Additional information
PfkA is a [wiki|moonlighting proteins|moonlighting protein] [Pubmed|19193632]
extensive information on the structure and enzymatic properties of PfkA can be found at [http://www.proteopedia.org/wiki/index.php/Phosphofructokinase_(PFK) Proteopedia]
Expression and Regulation
Operons
genes
[gene|A7E31A5EBCF343B3A841E5F6E9CDE3CA5D1622EB|pfkA]-[gene|F76A03A71DADC32C7166E37B994EFED019FDF8A4|pyk]-[gene|1C6D935B36416327FE63CABC53440441E8563D7E|ytzA]
description
[Pubmed|11489127]
regulation
twofold induced by glucose [Pubmed|11489127]
Biological materials
Mutant
GP590 ([gene|A7E31A5EBCF343B3A841E5F6E9CDE3CA5D1622EB|pfkA]::''cat''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
GP595 ([gene|A7E31A5EBCF343B3A841E5F6E9CDE3CA5D1622EB|pfkA]::''erm''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
GP1744: BSB1 [gene|A7E31A5EBCF343B3A841E5F6E9CDE3CA5D1622EB|pfkA]::''aphA3'', available in [wiki|Jörg Stülke]' lab
GP1747: [gene|A7E31A5EBCF343B3A841E5F6E9CDE3CA5D1622EB|pfkA]::''aphA3'', available in [wiki|Jörg Stülke]' lab, [Pubmed|27708634]
BKE29190 ([gene|A7E31A5EBCF343B3A841E5F6E9CDE3CA5D1622EB|pfkA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCATTCACCTCAGC, downstream forward: _UP4_TAATGTACAGCTGAAGGCTG
BKK29190 ([gene|A7E31A5EBCF343B3A841E5F6E9CDE3CA5D1622EB|pfkA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCATTCACCTCAGC, downstream forward: _UP4_TAATGTACAGCTGAAGGCTG
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP87, available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP382]: pGP1266, available in [wiki|Jörg Stülke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP393, available in [wiki|Jörg Stülke]'s lab
for expression in ''B. subtilis'', in [wiki|pBQ200]: pGP1422, available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
FLAG-tag construct
GP1019 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP511 (in [protein|search|pAC6]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1720 (in [wiki|pBP43]), expression of '' pfkA-GFP''::''spc'' under the native promoter, available in [wiki|Jörg Stülke]'s lab [Pubmed|27708634]
labs
[wiki|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
References
Page visits: 10467
Time of last update: 2024-12-22 08:07:11
Author of last update: Jstuelk