yflA
168
general stress protein, similar to amino acid carrier protein, required for protection against paraquat stress
locus
BSU_07750
Molecular weight
50.48 kDa
pI
9.86
function
protection against paraquat stress
product
unknown
essential
no
synonyms
yflA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1115 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
844,770 846,185
The protein
Protein family
[wiki|Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family] (according to UniProt)
Structure
[PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 37% identity) [pubmed|30659158]
[AF|O34708]
Paralogous protein(s)
[protein|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT], [protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|glnT], [protein|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]
description
[pubmed|22383849]
regulatory mechanism
[protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]: repression, in [regulon|protein:5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
genes
[gene|EA62F1DF6BB9204BB7A6B0CC6A05F63D711A451F|yflD]-[gene|530844B354D43308CC8CB7DC30DB9731A5D698EB|yflB]-[gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]
description
[pubmed|22383849]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|26577401], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
GP3557 (Δ[gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::phleo), available in [wiki|Jörg Stülke]'s lab
MGNA-C257 (yflA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2255 NBRP B. subtilis, Japan]
BKE07750 ([gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07750 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATCCCATCACCCTTTTTTC, downstream forward: _UP4_TCCTAGTGTTTTGCTTTTGC
BKK07750 ([gene|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCATCACCCTTTTTTC, downstream forward: _UP4_TCCTAGTGTTTTGCTTTTGC
References
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Time of last update: 2024-12-22 10:20:44
Author of last update: MBenda