thiI
168
sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA
locus
BSU_29580
Molecular weight
40.17 kDa
pI
9.66
function
biosynthesis of 4-thiouridine in tRNA
product
sulfuryl transferase
essential
no
synonyms
thiI, ytbJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0301 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,025,748 3,026,953
The protein
Catalyzed reaction/ biological activity
receives a sulfuryl group from [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ] and transfers it to uridine at position 8 of tRNA [Pubmed|22773787]
[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] --> [ThiI sulfur-carrier protein]-L-cysteine + 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] --> [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase] (according to UniProt)
Protein family
thiI family (single member, according to UniProt)
[wiki|Domains]
THUMP domain (aa 60-165) (according to UniProt)
Structure
[PDB|4KR6] (ThiI-RNA complex from Thermotoga maritima) [pubmed|24705700]
[PDB|2C5S] (from B. anthracis) [pubmed|16343540]
[AF|O34595]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT
BKK29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT
References
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Time of last update: 2024-12-29 20:42:23
Author of last update: Melvin.boenninger