nagZ
168
N-acetylglucosaminidase
locus
BSU_01660
Molecular weight
70.40 kDa
pI
9.76
function
cell wall recycling
product
N-acetylglucosaminidase
essential
no
ec
3.2.1.52
synonyms
nagZ, yzbA, ybbD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1472 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
186,452 188,380
Phenotypes of a mutant
increased autolysis [Pubmed|20400549]
The protein
Catalyzed reaction/ biological activity
cleaves muropeptides derived from peptidoglycan, but not peptidoglycan itself [Pubmed|20400549]
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides (according to UniProt)
Protein family
glycosyl hydrolase 3 family (single member, according to UniProt)
Structure
[PDB|3BMX] [Pubmed|20826810]
[AF|P40406]
[wiki|Localization]
secreted (with signal peptide), remains to some extent cell wall-associated [Pubmed|20400549]
Additional information
The gene is mis-annotated in KEGG as an ortholog of beta-N-acetylhexosaminidase EC 3.2.1.52. It is marked in MetaCyc as similar to beta-hexosaminidase. No EC annotation is available in Swiss-ProtSwiss-Prot.supporting the annotation is available. [Pubmed|19935659]
Expression and Regulation
Operons
genes
[gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|namZ]
description
[Pubmed|22383849]
regulation
expressed in late exponential and early stationary phase [Pubmed|20400549]
regulatory mechanism
[protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]: repression, [pubmed|30038046], in [regulon|protein:125872506C400CB6B3DAEA8B5130B064DFBA4494|murR regulon]
Biological materials
Mutant
available in [wiki|Christoph Mayer]'s lab [Pubmed|20400549]
BKE01660 ([gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGAAACTTCCTCCTATC, downstream forward: _UP4_AGACCGCTTTAATAAGGAGG
BKK01660 ([gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGAAACTTCCTCCTATC, downstream forward: _UP4_AGACCGCTTTAATAAGGAGG
References
Reviews
Original Publications
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Time of last update: 2024-12-29 01:08:35
Author of last update: Jstuelk