bcrC
168
undecaprenyl pyrophosphate phosphatase, produces the carrier lipid [metabolite|Und-P] for cell wall synthesis (major player), secondary bacitracin resistance determinant
locus
BSU_36530
Molecular weight
21.58 kDa
pI
9.45
function
cell wall synthesis, resistance to bacitracin and oxidative stress
product
undecaprenyl pyrophosphate phosphatase
essential
no
ec
3.6.1.27
synonyms
bcrC, ywoA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0671 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,758,547 3,759,128
Phenotypes of a mutant
5-fold increased sensitivity to bacitracin [Pubmed|26815905]
reduced growth rate [Pubmed|26815905]
increased susceptibility to rare earth elements [Pubmed|22904278]
a ''[gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]'' ''[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]'' double mutant is not viable (unless ''[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]'' is artificially expressed) [Pubmed|27528508]
poor growth of a [gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC] [gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM] double mutant [pubmed|39185233]
poor growth of a [gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC] [gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV] double mutant [pubmed|39185233]
inactivation suppresses the essentiality of [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|39185233]
The protein
Catalyzed reaction/ biological activity
undecaprenyl pyrophosphate - undecaprenyl phosphate
di-trans,octa-cis-undecaprenyl diphosphate + H2O --> di-trans,octa-cis-undecaprenyl phosphate + H+ + phosphate (according to UniProt)
Protein family
BcrC/YbjG family (single member, according to UniProt)
Structure
[AF|P94571]
Effectors of protein activity
inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
Additional information
there is a second undecaprenyl pyrophosphate phosphatase in ''B. subtilis'', [protein|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]. However, [protein|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP] seems to play only a minor role. [Pubmed|22904278]
Expression and Regulation
Operons
genes
[gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]
description
[pubmed|12850135]
regulation
expression activated by glucose (3.3 fold) [Pubmed|12850135]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [Pubmed|18156261], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|11866510], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|12399481], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
[protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]: sigma factor, in [regulon|protein:D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV regulon]
Biological materials
Mutant
MGNA-A214 (ywoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/214 NBRP B. subtilis, Japan]
BKE36530 ([gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATAATCACCTTTTACAT, downstream forward: _UP4_TAAGAAAGACAAAAGCCGGC
BKK36530 ([gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATAATCACCTTTTACAT, downstream forward: _UP4_TAAGAAAGACAAAAGCCGGC
References
Reviews
Original Publications
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Time of last update: 2024-12-27 20:04:04
Author of last update: Jstuelk