bcd

bcd
168

valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase

locus
BSU_24080
Molecular weight
39.83 kDa
pI
4.94
Protein length
364 aaSequenceBlast
Gene length
1095 bpSequenceBlast
function
utilization of branched-chain keto acids
product
valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase
essential
no
ec
1.4.1.9
synonyms
bcd, bkd, yqiT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0334 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,502,659  2,503,753
Phenotypes of a mutant
premature spore germination due to valine accumulation, activation of germination via [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA]-[protein|5FF739B8C087207039BB6DC293EE77F59337ED0B|gerAB]-[protein|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC] [pubmed|38564667]
The protein
Catalyzed reaction/ biological activity
L-leucine + H2O + NAD+ --> 4-methyl-2-oxopentanoate + NH4+ + NADH + H+ (according to UniProt)
Protein family
Glu/Leu/Phe/Val dehydrogenases family (with [protein|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|gudB] and [protein|56CBEBCFEF5CFB4A0175498338C7AF2F45EAA3E3|rocG], according to UniProt)
Structure
[PDB|1LEH] (from '' Lysinibacillus sphaericus'', 71% identity, 82% similarity) [Pubmed|8591046]
[AF|P54531]
Expression and Regulation
Operons
genes
[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
description
[Pubmed|12823818]
regulation
induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]
regulatory mechanism
[protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]: activation, [Pubmed|10094682], in [regulon|protein:4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|10094682], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|10094682], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
Open in new tab

[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]→[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]

2024-12-20 16:13:57

ghost

87

7b8921dc3f8fdb775067a1f92187895088ac1126

0BF3F61A9229E387986A64E3D2F9F647D24EA48A

Biological materials
Mutant
MGNA-C427 (yqiT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2425 NBRP B. subtilis, Japan]
a ''bcd'' mutant and a ''[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd] [gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ilvE] [gene|8163996FCB9D02F771E7A04A91D5720027260F12|ilvK]'' triple mutant are available in [wiki|Linc Sonenshein]'s lab
BKE24080 ([gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAAAGTTCCATGTTCGTTC,  downstream forward: _UP4_CGTTAAGAAATTGATCTGGA
BKK24080 ([gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAAAGTTCCATGTTCGTTC,  downstream forward: _UP4_CGTTAAGAAATTGATCTGGA
References
Reviews
7705101
Original Publications
12427936,15241682,10094682,12823818,24843172,30993221,38564667

A52E50104B18D0A8518218C52D9CC36FDDB29AAF

Page visits: 4141

Time of last update: 2024-12-26 02:26:01

Author of last update: Jstuelk