hmoA
168
heme monooxygenase
locus
BSU_07150
Molecular weight
14.57 kDa
pI
9.13
function
degradation of heme
product
heme monooxygenase
essential
no
synonyms
hmoA, yetG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2329 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
785,113 785,490
The protein
Catalyzed reaction/ biological activity
binds hemin ''in vitro'' with ~1:1 stoichiometry and degrades hemin in the presence of an electron donor [Pubmed|21873409]
heme b + 3 O2 + 3 reduced [NADPH—hemoprotein reductase] --> biliverdin IXα + CO + Fe2+ + H+ + 3 H2O + 3 oxidized [NADPH—hemoprotein reductase] (according to UniProt)
Protein family
antibiotic biosynthesis monooxygenase family (with [protein|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB], according to UniProt)
[wiki|Domains]
[wiki|ABM domain] (aa 2-95) (according to UniProt)
Structure
[PDB|1TZ0] (from [gene|search|B. cereus], 57% identity)
[AF|O31534]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A4E1A1FA886980C863919DA011AC1EEDDBD74967|hmoA]
description
(based on genomic context) null
regulation
induced by iron limitation ([protein|search|Fur]) [Pubmed|21873409]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [Pubmed|21873409], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
Biological materials
Mutant
MGNA-B458 (yetG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1457 NBRP B. subtilis, Japan]
BKE07150 ([gene|A4E1A1FA886980C863919DA011AC1EEDDBD74967|hmoA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTCTCACTCCAATCA, downstream forward: _UP4_TAAAAAGCTTGCGCCTTGCT
BKK07150 ([gene|A4E1A1FA886980C863919DA011AC1EEDDBD74967|hmoA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTCTCACTCCAATCA, downstream forward: _UP4_TAAAAAGCTTGCGCCTTGCT
References
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Time of last update: 2024-12-26 20:58:28
Author of last update: Jstuelk