ddl
168
D-alanine-D-alanine ligase
locus
BSU_04560
Molecular weight
38.97 kDa
pI
4.58
function
peptidoglycan precursor biosynthesis
product
D-alanine-D-alanine ligase
essential
yes
ec
6.3.2.4
synonyms
ddl, ddlA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1181 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
508,248 509,312
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + 2 D-alanine --> ADP + phosphate + D-alanyl-D-alanine (according to UniProt)
Protein family
D-alanine--D-alanine ligase family (single member, according to UniProt)
[wiki|Domains]
[wiki|ATP-grasp doamin] (aa 133-338) (according to UniProt)
Structure
[PDB|2YZG] (from ''Thermus thermophilus hb8 mutant'', 40% identity, 53% similarity)
[AF|P96612]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A47DBF8546E0F726A0FE58C21D3F28150290DF7F|ddl]-[gene|EC9F1D4F1863E53A818A51B0E16B201A251F077E|murF]
description
[Pubmed|22383849]
regulation
expression is reduced in a [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV] mutant [Pubmed|21926231]
the leader mRNA is processed upstream of [gene|A47DBF8546E0F726A0FE58C21D3F28150290DF7F|ddl] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]: sigma factor, in [regulon|protein:D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV regulon]
Biological materials
Mutant
MGNA-C104 (ddlA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2102 NBRP B. subtilis, Japan]
References
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Time of last update: 2024-12-30 15:58:20
Author of last update: Melvin.boenninger