yugS
168
spore membrane protein, similar to magnesium exporter
locus
BSU_31300
Molecular weight
49.33 kDa
pI
5.13
function
unknown
product
unknown
essential
no
synonyms
yugS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,216,163 3,217,452
The protein
Protein family
[wiki|UPF0053 family](according to UniProt)
[wiki|Domains]
[wiki|CNNM transmembrane domain] (aa 1-201) (according to UniProt)
[wiki|DUF21 domain] (4-201)
2 [wiki|CBS domain]s (aa 220-281, aa 284-341) (according to UniProt)
Structure
[PDB|4HG0] (CorC from E. coli, corresponds to the [wiki|CBS domain]s and the C-terminal transporter-associated domain)
[AF|O05241]
Paralogous protein(s)
[protein|B2E9D026B668C8530C406353E3400059388566FB|yhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA], [protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB], [protein|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]
[wiki|Localization]
cell membrane [pubmed|30602489]
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-A632 (yugS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/632 NBRP B. subtilis, Japan]
BKE31300 ([gene|A459410312A926365B45CEB4694DE399B38820A2|yugS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAAGCCCTTACCATA, downstream forward: _UP4_TGATAAAAAACAGCCGGGGA
BKK31300 ([gene|A459410312A926365B45CEB4694DE399B38820A2|yugS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAAGCCCTTACCATA, downstream forward: _UP4_TGATAAAAAACAGCCGGGGA
Expression vectors
pGP2936 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3466 (214-345aa) (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3471 (214-345aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-21 21:06:57
Author of last update: LKrueger