forC2

forC2
168

formate:menadione oxidoreductase (with [protein|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]), catalytic subunit

locus
BSU_27220
Molecular weight
108.57 kDa
pI
5.16
Protein length
Gene length
function
unknown
product
formate:menadione oxidoreductase
essential
no
ec
1.2.1.2
synonyms
yrhE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3383 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,781,209  2,784,151
The protein
Catalyzed reaction/ biological activity
formate + NAD+ --> CO2 + NADH (according to UniProt)
Protein family
C-terminal part: [wiki|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)
[wiki|Domains]
2Fe-2S ferredoxin-type domain (aa 5-81) (according to UniProt)
4Fe-4S His(Cys)3-ligated-type domain (aa 81-121) (according to UniProt)
2 [wiki|4Fe-4S ferredoxin-type domain]s (aa 144-171, aa 187-216) (according to UniProt)
[wiki|4Fe-4S Mo/W bis-MGD-type domain] (aa 263-319) (according to UniProt)
[wiki|Cofactors]
4 4Fe-4S cluster [pubmed|34748728]
1 2FE-2S cluster [pubmed|34748728]
molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) [pubmed|34748728]
menachinone-7 [pubmed|34748728]
Structure
[PDB|2FUG] (from Thermus thermophilus, 23% identity) [pubmed|16469879]
[AF|Q795Y4]
Modification
Fe-S cluster [pubmed|29292548]
Paralogous protein(s)
[protein|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]
Expression and Regulation
Operons
genes
[gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]-[gene|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]
description
[Pubmed|21815947]
additional information
term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|search|yrhE] [Pubmed|27120414]
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[gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]→[gene|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]

2024-10-30 17:03:59

Jstuelk

94

006C568235E4698DA2A79A779E974CDCC2C7AEDB

44A374BB2C1503068B122EAB1734F1DCA467F97C

Biological materials
Mutant
MGNA-A160 (yrhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/160 NBRP B. subtilis, Japan]
BKE27220 ([gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA,  downstream forward: _UP4_GATTAAACGATAGGAGGAAA
BKK27220 ([gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA,  downstream forward: _UP4_GATTAAACGATAGGAGGAAA
References
21815947,27120414,16469879,34748728

A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81

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Time of last update: 2024-11-02 01:13:41

Author of last update: Jstuelk