yrrN
168
tRNA uridine hydroxylase (in complex with [protein|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO])
locus
BSU_27350
Molecular weight
34.92 kDa
pI
4.64
function
introduction of 5-methoxyuridine modification in tRNA (U34)
product
tRNA uridine hydroxylase
essential
no
ec
null
synonyms
yrrN, trhP1
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0826 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,794,147 2,795,076
Phenotypes of a mutant
50% reduction in mo5U tRNA levels [pubmed|31358606]
The protein
Catalyzed reaction/ biological activity
hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with [protein|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by [protein|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]) [pubmed|31358606]
Protein family
peptidase U32 family (with [protein|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO], according to UniProt)
Structure
[AF|O32035]
Paralogous protein(s)
[protein|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]
Expression and Regulation
Operons
genes
[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]-[gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]-[gene|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]-[gene|A659D9894E777D8A99707CB750DF2918AEE06CE9|udk]-[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A008 (yrrN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/8 NBRP B. subtilis, Japan]
BKE27350 ([gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
BKK27350 ([gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
References
Research papers
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Time of last update: 2025-04-05 22:29:03
Author of last update: Jstuelk